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- W890274703 abstract "Publisher Summary This chapter discusses the principle and method of pteridine content of bacteria including photosynthetic bacteria. The 2-amino-4-hydroxypteridinyl radical is resistant to oxidation by alkaline permanganate. Most naturally occurring pteridines contain this radical in more-or-less modified form. Compounds with substituents at the 6-position yield the 6-carboxylic acid on oxidation; compounds in which the pyrazine ring of the pteridine moiety is reduced, are oxidized to the stable aromatic form; compounds with substituents on the pyrazine nitrogens probably lose these by oxidation and decarboxylation. Thus, the procedure converts all pteridines in the cell or culture fluid into a few derivatives that are easily separable from each other and other fluorescent permanganate oxidation products. Synthetic material is readily available for comparison, quantitatively and qualitatively. Cultures at appropriate stages are harvested by centrifugation, and the cells are washed three times with distilled water. The amounts of pteridines obtained are estimated on the original eluate by fluorescence in a standard fluorometer." @default.
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- W890274703 date "1971-01-01" @default.
- W890274703 modified "2023-09-27" @default.
- W890274703 title "[170] Pteridine content of bacteria including photosynthetic bacteria" @default.
- W890274703 doi "https://doi.org/10.1016/s0076-6879(71)18127-x" @default.
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