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- W8907300 abstract "This chapter discusses the in vitro immunization of dissociated murine spleen cells. A spleen cell suspension is cultured in plastic petri dishes at high cell density in the presence of antigen. The culture medium is supplemented with selected batches of fetal bovine serum. The cultures are incubated under low oxygen tension and with gentle agitation for a period of 4 to 6 days. The culture of unprimed lymphoid cells capable of responding to antigens seems to be a priori the most appropriate tool for the detailed study of induction and regulation of a humoral immune response. Ideally, in such an in vitro system, every constituent of the culture medium should be defined and the cell populations should be under the control of the experimenter. Any approach aiming at a better defined culture system will focus primarily either on the constituents of the medium and general culture conditions or on an exact definition of the cell populations cultured in a given medium. Neither approach has so far led to a satisfactory solution." @default.
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- W8907300 date "1979-01-01" @default.
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- W8907300 title "In Vitro Immunization of Dissociated Murine Spleen Cells" @default.
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- W8907300 doi "https://doi.org/10.1016/b978-0-12-442750-1.50029-2" @default.
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