Matches in SemOpenAlex for { <https://semopenalex.org/work/W89094098> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W89094098 endingPage "679" @default.
- W89094098 startingPage "671" @default.
- W89094098 abstract "We have compared actin-activated myosin ATPase activity, myosin binding to actin, and the velocity of myosin-induced actin sliding in order to understand the mechanism of myosin motility. In our in vitro assay, F-actin slides at a constant velocity, regardless of length. The F-actin could slide over myosin heads at KCl concentrations below a critical value (60 mM with myosin and HMM, 100 mM with S-1), and the sliding velocities were quite similar below the critical KCl concentration. However, at KCl concentrations close to the critical value, the sliding F-actin is attached to only one or a few particular points on the surface, each of which perhaps consists of a single head of myosin. The KATPase values for actin-activated ATPase were approximately 300 microM for S-1 and approximately 200 microM with HMM below the critical KCl concentration, and approximately 5,000 microM above the critical KCl concentration. This increase in KATPase is due to a drastic reduction in the binding affinity of myosin heads to F-actin, as determined by a proteolytic digestion method and direct observation by fluorescence microscopy. We also show that the Vmax of actin-activated myosin ATPase activity decreases steadily with increasing KCl concentration, even though the velocity of F-actin sliding remains unchanged. This result provides evidence that the ATPase activity is not necessarily linked to motility. We discuss possible models that do not require a tight coupling between myosin ATPase and motility." @default.
- W89094098 created "2016-06-24" @default.
- W89094098 creator A5014133166 @default.
- W89094098 creator A5016777509 @default.
- W89094098 creator A5063424073 @default.
- W89094098 creator A5084399391 @default.
- W89094098 date "1990-05-01" @default.
- W89094098 modified "2023-09-25" @default.
- W89094098 title "In Vitro Motility of Skeletal Muscle Myosin and Its Proteolytic Fragments1" @default.
- W89094098 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a123106" @default.
- W89094098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2144521" @default.
- W89094098 hasPublicationYear "1990" @default.
- W89094098 type Work @default.
- W89094098 sameAs 89094098 @default.
- W89094098 citedByCount "35" @default.
- W89094098 countsByYear W890940982014 @default.
- W89094098 countsByYear W890940982015 @default.
- W89094098 countsByYear W890940982017 @default.
- W89094098 countsByYear W890940982019 @default.
- W89094098 countsByYear W890940982021 @default.
- W89094098 countsByYear W890940982022 @default.
- W89094098 crossrefType "journal-article" @default.
- W89094098 hasAuthorship W89094098A5014133166 @default.
- W89094098 hasAuthorship W89094098A5016777509 @default.
- W89094098 hasAuthorship W89094098A5063424073 @default.
- W89094098 hasAuthorship W89094098A5084399391 @default.
- W89094098 hasConcept C105702510 @default.
- W89094098 hasConcept C12554922 @default.
- W89094098 hasConcept C125705527 @default.
- W89094098 hasConcept C15954220 @default.
- W89094098 hasConcept C181199279 @default.
- W89094098 hasConcept C185592680 @default.
- W89094098 hasConcept C18923293 @default.
- W89094098 hasConcept C23265538 @default.
- W89094098 hasConcept C2779959927 @default.
- W89094098 hasConcept C55493867 @default.
- W89094098 hasConcept C58207958 @default.
- W89094098 hasConcept C6997183 @default.
- W89094098 hasConcept C8035138 @default.
- W89094098 hasConcept C86803240 @default.
- W89094098 hasConcept C95444343 @default.
- W89094098 hasConceptScore W89094098C105702510 @default.
- W89094098 hasConceptScore W89094098C12554922 @default.
- W89094098 hasConceptScore W89094098C125705527 @default.
- W89094098 hasConceptScore W89094098C15954220 @default.
- W89094098 hasConceptScore W89094098C181199279 @default.
- W89094098 hasConceptScore W89094098C185592680 @default.
- W89094098 hasConceptScore W89094098C18923293 @default.
- W89094098 hasConceptScore W89094098C23265538 @default.
- W89094098 hasConceptScore W89094098C2779959927 @default.
- W89094098 hasConceptScore W89094098C55493867 @default.
- W89094098 hasConceptScore W89094098C58207958 @default.
- W89094098 hasConceptScore W89094098C6997183 @default.
- W89094098 hasConceptScore W89094098C8035138 @default.
- W89094098 hasConceptScore W89094098C86803240 @default.
- W89094098 hasConceptScore W89094098C95444343 @default.
- W89094098 hasIssue "5" @default.
- W89094098 hasLocation W890940981 @default.
- W89094098 hasLocation W890940982 @default.
- W89094098 hasOpenAccess W89094098 @default.
- W89094098 hasPrimaryLocation W890940981 @default.
- W89094098 hasRelatedWork W162570409 @default.
- W89094098 hasRelatedWork W1912809366 @default.
- W89094098 hasRelatedWork W1981127731 @default.
- W89094098 hasRelatedWork W1994466407 @default.
- W89094098 hasRelatedWork W2008702688 @default.
- W89094098 hasRelatedWork W2052841776 @default.
- W89094098 hasRelatedWork W2166185752 @default.
- W89094098 hasRelatedWork W2470311424 @default.
- W89094098 hasRelatedWork W2584251531 @default.
- W89094098 hasRelatedWork W2966272313 @default.
- W89094098 hasVolume "107" @default.
- W89094098 isParatext "false" @default.
- W89094098 isRetracted "false" @default.
- W89094098 magId "89094098" @default.
- W89094098 workType "article" @default.