Matches in SemOpenAlex for { <https://semopenalex.org/work/W89155536> ?p ?o ?g. }
- W89155536 endingPage "430" @default.
- W89155536 startingPage "385" @default.
- W89155536 abstract "Nuclei of amphibian oocytes (“germinal vesicles”) can grow to considerable size. For example, the nucleus of a mature Xenopus laevis oocyte has a diameter of about 0.5 mm and, after isolation, can be seen with the naked eye. This exceptional size (for comparison, the diameter of somatic cell nuclei usually ranges from 5 to 30 μm), the ease and rapidity with which they can be manually isolated, and the occurrence of lampbrush chromosomes and amplified nucleoli makes amphibian nuclei choice material not only for studies of chromosome structure and genetic activity at different levels of resolution, but also for analysis of the biochemical composition of individual nuclei and nuclear components as well. A single manually isolated nucleus from an amphibian oocyte is sufficient to analyze its major protein constituents by gel electrophoresis, and a few nuclei provide sufficient RNA for analysis by gel electrophoresis or electron microscopic spreading methods. In addition, the size of amphibian oocyte nuclei greatly facilitates the introduction of certain substances by microinjection. In fact, Xenopus oocytes are presently used in numerous laboratories as “living test tubes” to study the expression of cloned DNA sequences after microinjection into their nuclei with the aim of identifying those DNA sequences necessary for transcriptional initiation and termination events, as well as the factors involved in gene regulation." @default.
- W89155536 created "2016-06-24" @default.
- W89155536 creator A5049919211 @default.
- W89155536 creator A5089511485 @default.
- W89155536 date "1985-01-01" @default.
- W89155536 modified "2023-09-25" @default.
- W89155536 title "Functional Organization of the Amphibian Oocyte Nucleus" @default.
- W89155536 cites W120997348 @default.
- W89155536 cites W1459148598 @default.
- W89155536 cites W1488947141 @default.
- W89155536 cites W1547738298 @default.
- W89155536 cites W1576910771 @default.
- W89155536 cites W1601236940 @default.
- W89155536 cites W1964849116 @default.
- W89155536 cites W1965420151 @default.
- W89155536 cites W1965609818 @default.
- W89155536 cites W1970036299 @default.
- W89155536 cites W1970234431 @default.
- W89155536 cites W1971122464 @default.
- W89155536 cites W1972005309 @default.
- W89155536 cites W1975484124 @default.
- W89155536 cites W1975589498 @default.
- W89155536 cites W1978671755 @default.
- W89155536 cites W1979725861 @default.
- W89155536 cites W1980265594 @default.
- W89155536 cites W1982445736 @default.
- W89155536 cites W1982601116 @default.
- W89155536 cites W1983159345 @default.
- W89155536 cites W1985753454 @default.
- W89155536 cites W1986425554 @default.
- W89155536 cites W1989313376 @default.
- W89155536 cites W1993391472 @default.
- W89155536 cites W1995635845 @default.
- W89155536 cites W1998965262 @default.
- W89155536 cites W2000119709 @default.
- W89155536 cites W2000982290 @default.
- W89155536 cites W2003266654 @default.
- W89155536 cites W2003863219 @default.
- W89155536 cites W2007619188 @default.
- W89155536 cites W2015128173 @default.
- W89155536 cites W2015276401 @default.
- W89155536 cites W2018327189 @default.
- W89155536 cites W2020214400 @default.
- W89155536 cites W2020538144 @default.
- W89155536 cites W2021069133 @default.
- W89155536 cites W2021149998 @default.
- W89155536 cites W2021839313 @default.
- W89155536 cites W2025510213 @default.
- W89155536 cites W2027531387 @default.
- W89155536 cites W2032303982 @default.
- W89155536 cites W2035756674 @default.
- W89155536 cites W2036695592 @default.
- W89155536 cites W2039296143 @default.
- W89155536 cites W2040784874 @default.
- W89155536 cites W2042570087 @default.
- W89155536 cites W2043073648 @default.
- W89155536 cites W2043603714 @default.
- W89155536 cites W2046168172 @default.
- W89155536 cites W2046422192 @default.
- W89155536 cites W2049921961 @default.
- W89155536 cites W2055833890 @default.
- W89155536 cites W2058378934 @default.
- W89155536 cites W2058795573 @default.
- W89155536 cites W2060147939 @default.
- W89155536 cites W2061033217 @default.
- W89155536 cites W2069041747 @default.
- W89155536 cites W2074656438 @default.
- W89155536 cites W2079187231 @default.
- W89155536 cites W2084815098 @default.
- W89155536 cites W2088521046 @default.
- W89155536 cites W2089482316 @default.
- W89155536 cites W2099070975 @default.
- W89155536 cites W2104404316 @default.
- W89155536 cites W2137125182 @default.
- W89155536 cites W2152528574 @default.
- W89155536 cites W2158336385 @default.
- W89155536 cites W2170275232 @default.
- W89155536 cites W2265406928 @default.
- W89155536 cites W2315479748 @default.
- W89155536 cites W2318908212 @default.
- W89155536 cites W2505574320 @default.
- W89155536 cites W2886141486 @default.
- W89155536 cites W4243958980 @default.
- W89155536 cites W82455627 @default.
- W89155536 cites W2038975304 @default.
- W89155536 doi "https://doi.org/10.1007/978-1-4615-6814-8_9" @default.
- W89155536 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2481470" @default.
- W89155536 hasPublicationYear "1985" @default.
- W89155536 type Work @default.
- W89155536 sameAs 89155536 @default.
- W89155536 citedByCount "7" @default.
- W89155536 crossrefType "book-chapter" @default.
- W89155536 hasAuthorship W89155536A5049919211 @default.
- W89155536 hasAuthorship W89155536A5089511485 @default.
- W89155536 hasBestOaLocation W891555362 @default.
- W89155536 hasConcept C104317684 @default.
- W89155536 hasConcept C129536746 @default.
- W89155536 hasConcept C134305767 @default.