Matches in SemOpenAlex for { <https://semopenalex.org/work/W89204057> ?p ?o ?g. }
- W89204057 abstract "Mammalian oocytes are arrested at prophase I of meiosis until a preovulatory surge of LH stimulates them to resume meiosis. Prior to the LH surge, high levels of cAMP within the oocyte maintain meiotic arrest; this cAMP is generated in the oocyte through the activity of the constitutively active, GRsR-coupled receptor, G-protein-coupled receptor 3 (GPR3) or GPR12. Activated GPRs are typically targeted for desensitization through receptor-mediated endocytosis, but a continuously high level of cAMP is needed for meiotic arrest. The aim of this study was to examine whether receptor-mediated endocytosis occurs in the mouse oocyte and whether this could affect the maintenance of meiotic arrest. We found that constitutive endocytosis occurs in the mouse oocyte. Inhibitors of receptor-mediated endocytosis, monodansylcadaverine and dynasore, inhibited the formation of early endosomes and completely inhibited spontaneous meiotic resumption. A red fluorescent protein-tagged GPR3 localized in the plasma membrane and within early endosomes in the oocyte, demonstrating that GPR3 is endocytosed. However, overexpression of G-arrestin-2 had only a modest effect on stimulating meiotic resumption, suggesting that these proteins do not play a major role in GPR3 endocytosis. Inhibition of endocytosis elevated cAMP levels within oocytes, suggesting that there is an accumulation of GPR3 at the plasma membrane. These results show that endocytosis occurs in the oocyte, leading to a decrease in cAMP production, and suggest that there is a balance between cAMP" @default.
- W89204057 created "2016-06-24" @default.
- W89204057 creator A5018677291 @default.
- W89204057 date "2013-01-01" @default.
- W89204057 modified "2023-09-27" @default.
- W89204057 title "Studies on Mouse Oocyte Meiotic Arrest and Maturation" @default.
- W89204057 cites W1492038278 @default.
- W89204057 cites W1507084693 @default.
- W89204057 cites W1541025915 @default.
- W89204057 cites W1541561513 @default.
- W89204057 cites W1548284562 @default.
- W89204057 cites W1550298527 @default.
- W89204057 cites W1559767829 @default.
- W89204057 cites W1580968401 @default.
- W89204057 cites W158763770 @default.
- W89204057 cites W1600955774 @default.
- W89204057 cites W1604209299 @default.
- W89204057 cites W1604407960 @default.
- W89204057 cites W1664200544 @default.
- W89204057 cites W1718009280 @default.
- W89204057 cites W1769810164 @default.
- W89204057 cites W1795151741 @default.
- W89204057 cites W1909076506 @default.
- W89204057 cites W1945187946 @default.
- W89204057 cites W1961999388 @default.
- W89204057 cites W1963701928 @default.
- W89204057 cites W1963899666 @default.
- W89204057 cites W1966135676 @default.
- W89204057 cites W1966244470 @default.
- W89204057 cites W1966263664 @default.
- W89204057 cites W1966389585 @default.
- W89204057 cites W1966434897 @default.
- W89204057 cites W1967586250 @default.
- W89204057 cites W1969030895 @default.
- W89204057 cites W1969041583 @default.
- W89204057 cites W1970318944 @default.
- W89204057 cites W1970853792 @default.
- W89204057 cites W1971022983 @default.
- W89204057 cites W1971748110 @default.
- W89204057 cites W1971908496 @default.
- W89204057 cites W1972130588 @default.
- W89204057 cites W1973197643 @default.
- W89204057 cites W1973433000 @default.
- W89204057 cites W1974560738 @default.
- W89204057 cites W1974887924 @default.
- W89204057 cites W1975180353 @default.
- W89204057 cites W1975425719 @default.
- W89204057 cites W1976377553 @default.
- W89204057 cites W1978416664 @default.
- W89204057 cites W1978570830 @default.
- W89204057 cites W1979707418 @default.
- W89204057 cites W1979815209 @default.
- W89204057 cites W1980753190 @default.
- W89204057 cites W1981236576 @default.
- W89204057 cites W1983229130 @default.
- W89204057 cites W1983604049 @default.
- W89204057 cites W1983757092 @default.
- W89204057 cites W1984212762 @default.
- W89204057 cites W1986140299 @default.
- W89204057 cites W1987720030 @default.
- W89204057 cites W1988186145 @default.
- W89204057 cites W1989445395 @default.
- W89204057 cites W1989710827 @default.
- W89204057 cites W1990522517 @default.
- W89204057 cites W1991138299 @default.
- W89204057 cites W1992335354 @default.
- W89204057 cites W1993054972 @default.
- W89204057 cites W1993937267 @default.
- W89204057 cites W1994898947 @default.
- W89204057 cites W1996193406 @default.
- W89204057 cites W1998703307 @default.
- W89204057 cites W1999470156 @default.
- W89204057 cites W1999558966 @default.
- W89204057 cites W1999767086 @default.
- W89204057 cites W2002841191 @default.
- W89204057 cites W2004674628 @default.
- W89204057 cites W2004755511 @default.
- W89204057 cites W2005682259 @default.
- W89204057 cites W2008977389 @default.
- W89204057 cites W2009821764 @default.
- W89204057 cites W2010131812 @default.
- W89204057 cites W2010782393 @default.
- W89204057 cites W2010932551 @default.
- W89204057 cites W2012641207 @default.
- W89204057 cites W2013229182 @default.
- W89204057 cites W2013967645 @default.
- W89204057 cites W2015557754 @default.
- W89204057 cites W2015893386 @default.
- W89204057 cites W2016064399 @default.
- W89204057 cites W2017214646 @default.
- W89204057 cites W2018831893 @default.
- W89204057 cites W2020137180 @default.
- W89204057 cites W2020329015 @default.
- W89204057 cites W2022750564 @default.
- W89204057 cites W2023601927 @default.
- W89204057 cites W2023724239 @default.
- W89204057 cites W2024460610 @default.
- W89204057 cites W2025472785 @default.
- W89204057 cites W2025599241 @default.
- W89204057 cites W2025976079 @default.