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• W896091808 abstract "Neutrophils prevail during the first hours after infection by Leishmania and therefore represent one of the first cell populations to migrate to the site of the infection, representing about 80 to 90% of the cellular infiltrate and, thus can contribute to a protective immune response on infection by this parasite. However, different studies have shown that neutrophils may assist in establishment of infection, via inactivation of macrophage antileishmanial mechanisms. Whereas most studies address the infection by Leishmania major, it is of great importance to study the role of neutrophils in other species of Leishmania. In our study, we evaluated in vitro response of neutrophils in the presence of different Leishmania species. For this, neutrophils harvested from bone marrow of C57BL/6 mice and then purified by Percoll gradient were cultured in the presence of L. amazonensis, L. braziliensis or L. major for 3 hours at 37°C / 5%CO2. Initially, we evaluated the viability of neutrophils infected by parasites of the genus Leishmania. The results showed that the percentage of viable cells did not change, regardless of the species of Leishmania used in the infection. We, then, evaluated the phagocytic ability by stainning parasites with carboxyfluorescein succinimidyl ester (CFSE) and the results showed a higher percentage of neutrophil phagocytosis in the presence of L. braziliensis when compared to L. amazonensis or L. major. Subsequently, we assessed the functional activation of these cells through the evaluation of nitric oxide (NO) and reactive oxygen species (ROS) intracellular expression by flow cytometry. Our results showed an increased expression of both NO and ROS by neutrophil infected with L. braziliensis when compared to L. amazonensis or L. major. In addition, we evaluated the expression of activation molecules (CD11b/CD49d/CD62L and TLR2/TLR4/TLR9) and the results showed a decreased expression of CD62L and an increase in the expression of TLR2 and TLR9 on neutrophils infected by three Leishmania species. Moreover, we observed an increase in expression of TLR4 in neutrophils infected by L. braziliensis, when compared to the population without stimulation, as well as increased TLR2 when compared with the other species. In addition, we evaluated the levels of chemokines (MCP-1, MCP-3, MIP-1α, MIP-1β and RANTES) in the culture supernatant and the results showed that only neutrophils infected by L. amazonensis or L. braziliensis produced MIP-1α. Moreover, independently of the stimulus, neutrophils were able to produce MIP-1β. Finally, the results of IL-12, TNF-α, IL-10 and TGF-β intracellular expression showed increased levels of TNF-α in neutrophils infected by the three species of the parasite. Additionally, this same result was observed in neutrophils expression of IL-10 infected by L. amazonensis or L. braziliensis and, in neutrophils expression IL-12p40 infected by L. amazonensis, when compared to non stimulated neutrophils. No difference was observed for the cytokine TGF-β in neutrophils infected by the three species of the parasite. Together, these data demonstrated that neutrophils may have an important role in the control of infection, as well as establishing and directing a protective response. Furthermore, it can be suggested that L. braziliensis was more effective in inducing a profile activation and expression of inflammatory mediators when compared to other species, suggesting a possible mechanism of control neutrophils triggered by the infection." @default.
• W896091808 created "2016-06-24" @default.
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• W896091808 date "2013-01-01" @default.
• W896091808 modified "2023-09-24" @default.
• W896091808 title "Avaliação, in vitro, da resposta imune de neutrófilos de camundongos C57BL/6 estimulados com diferentes espécies de Leishmania." @default.
• W896091808 hasPublicationYear "2013" @default.
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