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- W896330899 abstract "ABSTRACT Backscattered electrons(BSE) are generated at the impact of the primary electron beam on the specimen. BSE imagingprovides the compositional contrast to resolve chemical features of sectioned block-face. A focused ion beam(FIB) columncan be combined with a field emission scanning electron microscope (FESEM) to ensure a dual (or cross)-beam system(FIB-FESEM). Due to the milling of the specimen material by 10 to 100nm with the gallium ion beam, FIB-FESEM allowsthe serial block-face (SBF) imaging of plastic-embedded specimens with high z-axis resolution. After contrast inversion,BSE images are similar to transmitted electron images by transmission electron microscopy. As another means of SBFimaging, a specialized ultramirotome has been incorporated into the specimen chamber of FESEM (3View ® ). Internalstructures of plastic-embedded specimens can be serially revealed and analyzed by 3View ® with a large field of view tofacilitate three-dimensional reconstruction. These two SBF approaches by FESEM can be employed to unravel spatialassociation of(sub)cellular entities for a comprehensive understanding of complex biological systems.Keywords : 3View, Focused ion beam, Serial block-face, Tomography" @default.
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- W896330899 date "2011-01-01" @default.
- W896330899 modified "2023-09-23" @default.
- W896330899 title "Serial Block-Face Imaging by Field Emission Scanning Electron Microscopy" @default.
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