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- W8965275 abstract "Scanning ion conductance microscopy (SICM) offers a number of benefits for bothimaging and recording from biological samples. However, several fundamental issuesconcerning this technique, including the image resolution, remain to be clearly understood. Inthis thesis a model of SICM has been developed to address these issues. This model is quitegeneral and can accommodate arbitrary geometries for both the probe and the sample surface.Model simulations using simple geometries are in good agreement with known analyticalsolutions and experimental data. The simulations have therefore been extended to examine anumber of imaging issues, including the lateral resolution of SICM in response to a surface'step' and to a pair of adjacent objects. The probe’s interactions with features that are smallerthan, or comparable to, the size of the probe tip have also been investigated. Further, thescanning of sloped objects has also been examined. The results of these simulations suggestsome important considerations for scanning. The potential for extending the biological uses ofSICM has also been examined in the area of protein/peptide secretion. It is demonstrated thatSICM can be used to image secretory events from the Weibel-Palade bodies in endothelial cells.Further, to explore similar issues, on a smaller scale, the use of SICM to study neuropeptide Y(NPY) secretion has been examined. To this end a new preprohormone construct of NPY, whichallows visualisation of the location of secretory events, has been tested. This construct containsa region coding for the pH-sensitive ecliptic pHluorin, and is shown to be capable of reportingfusion events, without major disruption of the kinetics of secretion. Experiments using thisconstruct in conjunction with SICM are described and the potential for using SICM to provideinsight into peptide secretion is discussed." @default.
- W8965275 created "2016-06-24" @default.
- W8965275 creator A5084145941 @default.
- W8965275 date "2011-09-28" @default.
- W8965275 modified "2023-09-26" @default.
- W8965275 title "Scanning ion conductance microscopy : modelling and approaches to studying peptide secretion" @default.
- W8965275 hasPublicationYear "2011" @default.
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