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- W896747467 abstract "This chapter describes the methods for nuclear transplantation in amphibia. The reasons for developing the technique of nuclear transplantation are to test the genetic content of somatic cells and to analyze interactions between nucleus and cytoplasm. The chapter discusses potential applications of this technique. Among multicellular organisms, the large size and ready availability of the eggs and oocytes of amphibia give them great advantages for nuclear transplantation over those of other animals. The techniques are applicable to eggs and oocytes of Xenopus laevis. Minor differences in technique apply to eggs of Ram pipiens. Hormone injection causes fully grown oocytes to be released from the ovary, passed down the oviduct where they are surrounded by jelly, and released into the surrounding water. Eggs, unlike oocytes, can be fertilized and can commence development. The most useful applications of nuclear transplantation methods for investigating the function of chromosomal proteins involve multiple nuclear transfers and the injection of purified genes or chromosomal proteins." @default.
- W896747467 created "2016-06-24" @default.
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- W896747467 date "1977-01-01" @default.
- W896747467 modified "2023-10-14" @default.
- W896747467 title "Chapter 7 Methods for Nuclear Transplantation in Amphibia" @default.
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- W896747467 doi "https://doi.org/10.1016/s0091-679x(08)60096-5" @default.
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