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- W896898983 abstract "The successful application of solid-state NMR spectroscopy for structural study of biological macromolecules requires high spectral resolution. In presence of abundant H1 spins, the resolution of the prevailing C13 or N15 chemical shift encoding experiments critically depends on the availability of efficient and robust heteronuclear decoupling methods in addition to the use of high-field instrumentation and fast sample spinning. Robustness of the decoupling method towards alterations in amplitude/offset of radio frequency fields due to varying sample states is important to ensure recording of spectra with high resolution over long sampling periods for insensitive samples. Here, we present a phase-alternated refocused continuous-wave decoupling method offering better resolution, easier setup, and higher robustness than previous methods. Improved decoupling is in part ascribed to more efficient cancellation of the residual heteronuclear, H1–C13, dipolar coupling interactions which are induced by homonuclear, H1–H1, dipolar coupling interactions." @default.
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- W896898983 date "2015-08-01" @default.
- W896898983 modified "2023-09-25" @default.
- W896898983 title "Improving spectral resolution in biological solid-state NMR using phase-alternated rCW heteronuclear decoupling" @default.
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- W896898983 doi "https://doi.org/10.1016/j.cplett.2015.07.008" @default.
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