FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W89741002> ?p ?o ?g. }

• W89741002 endingPage "68" @default.
• W89741002 startingPage "59" @default.
• W89741002 abstract "Nucleic acid amplification is the basis for many molecular diagnostic assays. In these cases, the amplification product must be detected and analyzed, typically requiring extended workflow time, sophisticated equipment, or both. Here we present a novel method of amplification detection that harnesses the pH change resulting from amplification reactions performed with minimal buffering capacity. In loop-mediated isothermal amplification (LAMP) reactions, we achieved rapid (<30 min) and sensitive (<10 copies) visual detection using pH-sensitive dyes. Additionally, the detection can be performed in real time, enabling high-throughput or quantitative applications. We also demonstrate this visual detection for another isothermal amplification method (strand-displacement amplification), PCR, and reverse transcription LAMP (RT-LAMP) detection of RNA. The colorimetric detection of amplification presented here represents a generally applicable approach for visual detection of nucleic acid amplification, enabling molecular diagnostic tests to be analyzed immediately without the need for specialized and expensive instrumentation." @default.
• W89741002 created "2016-06-24" @default.
• W89741002 creator A5034315022 @default.
• W89741002 creator A5035914775 @default.
• W89741002 creator A5079083791 @default.
• W89741002 date "2015-02-01" @default.
• W89741002 modified "2023-10-18" @default.
• W89741002 title "Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes" @default.
• W89741002 cites W162279249 @default.
• W89741002 cites W1895298047 @default.
• W89741002 cites W1975801865 @default.
• W89741002 cites W1979974453 @default.
• W89741002 cites W1988659709 @default.
• W89741002 cites W1988929438 @default.
• W89741002 cites W1992176409 @default.
• W89741002 cites W2013139781 @default.
• W89741002 cites W2019786863 @default.
• W89741002 cites W2035792726 @default.
• W89741002 cites W2048255163 @default.
• W89741002 cites W2062237873 @default.
• W89741002 cites W2065098811 @default.
• W89741002 cites W2066385972 @default.
• W89741002 cites W2067737436 @default.
• W89741002 cites W2082133857 @default.
• W89741002 cites W2085839105 @default.
• W89741002 cites W2094672529 @default.
• W89741002 cites W2100538295 @default.
• W89741002 cites W2105275554 @default.
• W89741002 cites W2112493925 @default.
• W89741002 cites W2117240806 @default.
• W89741002 cites W2141633648 @default.
• W89741002 cites W2150186914 @default.
• W89741002 cites W2153350094 @default.
• W89741002 cites W81757823 @default.
• W89741002 doi "https://doi.org/10.2144/000114253" @default.
• W89741002 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25652028" @default.
• W89741002 hasPublicationYear "2015" @default.
• W89741002 type Work @default.
• W89741002 sameAs 89741002 @default.
• W89741002 citedByCount "427" @default.
• W89741002 countsByYear W897410022015 @default.
• W89741002 countsByYear W897410022016 @default.
• W89741002 countsByYear W897410022017 @default.
• W89741002 countsByYear W897410022018 @default.
• W89741002 countsByYear W897410022019 @default.
• W89741002 countsByYear W897410022020 @default.
• W89741002 countsByYear W897410022021 @default.
• W89741002 countsByYear W897410022022 @default.
• W89741002 countsByYear W897410022023 @default.
• W89741002 crossrefType "journal-article" @default.
• W89741002 hasAuthorship W89741002A5034315022 @default.
• W89741002 hasAuthorship W89741002A5035914775 @default.
• W89741002 hasAuthorship W89741002A5079083791 @default.
• W89741002 hasBestOaLocation W897410021 @default.
• W89741002 hasConcept C104317684 @default.
• W89741002 hasConcept C139713532 @default.
• W89741002 hasConcept C153911025 @default.
• W89741002 hasConcept C156385826 @default.
• W89741002 hasConcept C179786068 @default.
• W89741002 hasConcept C185592680 @default.
• W89741002 hasConcept C203014093 @default.
• W89741002 hasConcept C24107716 @default.
• W89741002 hasConcept C2777391075 @default.
• W89741002 hasConcept C2909633786 @default.
• W89741002 hasConcept C49105822 @default.
• W89741002 hasConcept C54355233 @default.
• W89741002 hasConcept C552990157 @default.
• W89741002 hasConcept C55493867 @default.
• W89741002 hasConcept C60635243 @default.
• W89741002 hasConcept C78063203 @default.
• W89741002 hasConcept C86803240 @default.
• W89741002 hasConceptScore W89741002C104317684 @default.
• W89741002 hasConceptScore W89741002C139713532 @default.
• W89741002 hasConceptScore W89741002C153911025 @default.
• W89741002 hasConceptScore W89741002C156385826 @default.
• W89741002 hasConceptScore W89741002C179786068 @default.
• W89741002 hasConceptScore W89741002C185592680 @default.
• W89741002 hasConceptScore W89741002C203014093 @default.
• W89741002 hasConceptScore W89741002C24107716 @default.
• W89741002 hasConceptScore W89741002C2777391075 @default.
• W89741002 hasConceptScore W89741002C2909633786 @default.
• W89741002 hasConceptScore W89741002C49105822 @default.
• W89741002 hasConceptScore W89741002C54355233 @default.
• W89741002 hasConceptScore W89741002C552990157 @default.
• W89741002 hasConceptScore W89741002C55493867 @default.
• W89741002 hasConceptScore W89741002C60635243 @default.
• W89741002 hasConceptScore W89741002C78063203 @default.
• W89741002 hasConceptScore W89741002C86803240 @default.
• W89741002 hasIssue "2" @default.
• W89741002 hasLocation W897410021 @default.
• W89741002 hasLocation W897410022 @default.
• W89741002 hasLocation W897410023 @default.
• W89741002 hasOpenAccess W89741002 @default.
• W89741002 hasPrimaryLocation W897410021 @default.
• W89741002 hasRelatedWork W1965010242 @default.
• W89741002 hasRelatedWork W2323826182 @default.
• W89741002 hasRelatedWork W2362076333 @default.
• W89741002 hasRelatedWork W2392015243 @default.

• next