FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W90048484> ?p ?o ?g. }

• W90048484 endingPage "715" @default.
• W90048484 startingPage "706" @default.
• W90048484 abstract "Abstract Human granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) exert multiple effects on the proliferation, differentiation, and function of myeloid lineage cells through their interaction with specific cell-surface receptors. There is a considerable degree of overlap in the biological effects of these two growth factors, but little is known about the mechanisms of postreceptor signal transduction. We have investigated the effects of GM-CSF and IL-3 on protein tyrosine-kinase activity in a human cell line, MO7E, which proliferates in response to either factor. Tyrosine- kinase activity was detected using immunoblotting with a monoclonal antibody (MoAb) specific for phosphotyrosine. GM-CSF and IL-3 were found to induce a nearly identical pattern of protein tyrosine phosphorylation using both one- and two-dimensional gel electrophoresis. Tyrosine phosphorylation of two cytosolic proteins in particular was increased more than 10-fold, a 93-Kd protein (pp93) and a 70-Kd protein (pp70). Tyrosine phosphorylation of pp93 and pp70 was observed within 1 minute, reached a maximum at 5 to 15 minutes, and gradually decreased thereafter. Other proteins of 150, 125, 63, 55, 42, and 36 Kd were also phosphorylated on tyrosine in response to both GM- CSF and IL-3, although to a lesser degree. Tyrosine phosphorylation was dependent on the concentration of GM-CSF over the range of 0.1 to 10 ng/mL and on IL-3 over the range of 1 to 30 ng/mL. Stimulation of MO7E cells with 12–0-tetradecanoyl-phorbol-13-acetate (TPA) or cytokines such as G-CSF, M-CSF, interleukin-1 (IL-1), interleukin-4 (IL-4), interleukin-6 (IL-6), interferon gamma, tumor necrosis factor (TNF), or transforming growth factor-beta (TGF-beta) did not induce tyrosine phosphorylation of pp93 or pp70, suggesting that these two phosphoproteins are specific for GM-CSF-or IL-3-induced activation. The extent and duration of phosphorylation of all the substrates were increased by pretreatment of cells with vanadate, an inhibitor of protein-tyrosine phosphatases. Importantly, culture of MO7E cells with vanadate (up to 10 mumol/L) resulted in a dose-dependent increase in GM- CSF-or IL-3-induced proliferation of up to 1.8-fold. These results suggest that tyrosine phosphorylation may be important for GM-CSF and IL-3 receptor-mediated signal transduction and that cell proliferation may be, at least partially, regulated by a balance between CSF-induced protein-tyrosine kinase activity and protein-tyrosine phosphatase activity." @default.
• W90048484 created "2016-06-24" @default.
• W90048484 creator A5033023115 @default.
• W90048484 creator A5039503690 @default.
• W90048484 creator A5051683488 @default.
• W90048484 creator A5055453369 @default.
• W90048484 creator A5055648823 @default.
• W90048484 creator A5066702054 @default.
• W90048484 date "1990-08-15" @default.
• W90048484 modified "2023-10-16" @default.
• W90048484 title "Signal transduction of the human granulocyte-macrophage colony- stimulating factor and interleukin-3 receptors involves tyrosine phosphorylation of a common set of cytoplasmic proteins" @default.
• W90048484 cites W1499420960 @default.
• W90048484 cites W1525342416 @default.
• W90048484 cites W1527111072 @default.
• W90048484 cites W1537772708 @default.
• W90048484 cites W1582473274 @default.
• W90048484 cites W1584755624 @default.
• W90048484 cites W1585663943 @default.
• W90048484 cites W1964846497 @default.
• W90048484 cites W1965165819 @default.
• W90048484 cites W1967643308 @default.
• W90048484 cites W1968610357 @default.
• W90048484 cites W1973312385 @default.
• W90048484 cites W1978972285 @default.
• W90048484 cites W1987757198 @default.
• W90048484 cites W1990350386 @default.
• W90048484 cites W2002623358 @default.
• W90048484 cites W2005388055 @default.
• W90048484 cites W2012681367 @default.
• W90048484 cites W2022653997 @default.
• W90048484 cites W2037434894 @default.
• W90048484 cites W2074498234 @default.
• W90048484 cites W2074571771 @default.
• W90048484 cites W2077047827 @default.
• W90048484 cites W2084424583 @default.
• W90048484 cites W2102367506 @default.
• W90048484 cites W2103174300 @default.
• W90048484 cites W2109202813 @default.
• W90048484 cites W2111295274 @default.
• W90048484 cites W2114918609 @default.
• W90048484 cites W2164755004 @default.
• W90048484 cites W2229587170 @default.
• W90048484 cites W2280040295 @default.
• W90048484 cites W2410918592 @default.
• W90048484 cites W3102875255 @default.
• W90048484 cites W984887640 @default.
• W90048484 doi "https://doi.org/10.1182/blood.v76.4.706.706" @default.
• W90048484 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2166606" @default.
• W90048484 hasPublicationYear "1990" @default.
• W90048484 type Work @default.
• W90048484 sameAs 90048484 @default.
• W90048484 citedByCount "290" @default.
• W90048484 countsByYear W900484842012 @default.
• W90048484 countsByYear W900484842017 @default.
• W90048484 countsByYear W900484842021 @default.
• W90048484 crossrefType "journal-article" @default.
• W90048484 hasAuthorship W90048484A5033023115 @default.
• W90048484 hasAuthorship W90048484A5039503690 @default.
• W90048484 hasAuthorship W90048484A5051683488 @default.
• W90048484 hasAuthorship W90048484A5055453369 @default.
• W90048484 hasAuthorship W90048484A5055648823 @default.
• W90048484 hasAuthorship W90048484A5066702054 @default.
• W90048484 hasBestOaLocation W900484841 @default.
• W90048484 hasConcept C101544691 @default.
• W90048484 hasConcept C11960822 @default.
• W90048484 hasConcept C153911025 @default.
• W90048484 hasConcept C170493617 @default.
• W90048484 hasConcept C180361614 @default.
• W90048484 hasConcept C195794163 @default.
• W90048484 hasConcept C202751555 @default.
• W90048484 hasConcept C2775960820 @default.
• W90048484 hasConcept C2776165026 @default.
• W90048484 hasConcept C2777553839 @default.
• W90048484 hasConcept C2779244956 @default.
• W90048484 hasConcept C42362537 @default.
• W90048484 hasConcept C50442881 @default.
• W90048484 hasConcept C55493867 @default.
• W90048484 hasConcept C62478195 @default.
• W90048484 hasConcept C85528070 @default.
• W90048484 hasConcept C86803240 @default.
• W90048484 hasConcept C87325107 @default.
• W90048484 hasConcept C95444343 @default.
• W90048484 hasConcept C97029542 @default.
• W90048484 hasConceptScore W90048484C101544691 @default.
• W90048484 hasConceptScore W90048484C11960822 @default.
• W90048484 hasConceptScore W90048484C153911025 @default.
• W90048484 hasConceptScore W90048484C170493617 @default.
• W90048484 hasConceptScore W90048484C180361614 @default.
• W90048484 hasConceptScore W90048484C195794163 @default.
• W90048484 hasConceptScore W90048484C202751555 @default.
• W90048484 hasConceptScore W90048484C2775960820 @default.
• W90048484 hasConceptScore W90048484C2776165026 @default.
• W90048484 hasConceptScore W90048484C2777553839 @default.
• W90048484 hasConceptScore W90048484C2779244956 @default.
• W90048484 hasConceptScore W90048484C42362537 @default.
• W90048484 hasConceptScore W90048484C50442881 @default.
• W90048484 hasConceptScore W90048484C55493867 @default.
• W90048484 hasConceptScore W90048484C62478195 @default.

• next