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- W90117349 abstract "The carotid bodies (CB) are the major arterial O2 chemoreceptors in mammals. The CB glomus cell response to hypoxia is mediated by multiple ion channels, including TASK-like background K+ channels, other K+ channels and voltage-gated calcium channels. In order to investigate the physiological role of TASK-like K+ channels in CB O2 sensing, we applied RNA interference technology by knocking down gene/protein expression and observing its functional effect on O2 sensing. Dissociated CB glomus cells from 14 day old rats were incubated overnight and treated with siRNA of TASK-1, TASK-3 or non-silencing siRNA with optimized transfection reagent. After cells were incubated for 48 hrs, gene expression was measured with real time PCR and protein expression was tested with immunostaining. Functional effects of siRNA exposure were assessed using fura-2 to measure the glomus cell intracellular calcium ([Ca2+]i) response to hypoxia. Results: TASK-3 siRNA reduced gene expression ~70%, while non-silencing siRNA had no effect. TASK-1siRNA exposure reduced TASK-1 protein expression (immunostaining) and reduced the glomus cell [Ca2+]i response to hypoxia ~70%, while non-silencing siRNA had no effect. These findings suggest that TASK-like K+ channels play an important role in the glomus cell response to hypoxia and RNA interference methods represent a feasible approach to exploring the role of specific ion channels in O2 CB sensing. (funded by University of Arkansas for Medical Sciences Dean’s Research Development Fund)." @default.
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- W90117349 date "2007-01-01" @default.
- W90117349 modified "2023-09-23" @default.
- W90117349 title "Modification of rat carotid body glomus cell O2 sensing by RNA interference method" @default.
- W90117349 doi "https://doi.org/10.1096/fasebj.21.6.lb113-d" @default.
- W90117349 hasPublicationYear "2007" @default.
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