Matches in SemOpenAlex for { <https://semopenalex.org/work/W906500352> ?p ?o ?g. }
- W906500352 abstract "Dopamine (DA) and noradrenalin (NA) are critically involved in multiple brain functions. NA and DA modulate neuronal functions via G protein coupled receptors and cAMP/PKA signaling. NA is released in the entire neocortex by axons of locus coeruleus (LC) neurons. DA projections to the neocortex, which originate in the ventral tegmental area, innervate more restricted areas. The aim of this project is to characterize the effect of exogenous and endogenously released NA and DA on cAMP/PKA signaling by using PKA sensitive fluorescent sensors. I showed PKA activation in pyramidal neurons in response to bath application of NA, isoproterenol, DA and SKF38393. Responses were observed throughout the cortex even in areas poorly innervated by DA fibers. Responses to NA and isoproterenol were inhibited by β-receptors antagonists while responses to DA and SKF38393 were blocked by a D1/D5 receptor antagonist. The negative contribution of D2-like and α2-adrenoceptor to PKA signaling was also demonstrated using specific antagonists. These results show a widespread distribution of functional NA and DA receptors in the neocortex that extends far beyond the territory of DA fiber innervations. The selective expression of the light activated Channelrhodopsin2 in NA neurons combined with cortical PKA imaging allowed demonstrating that release of endogenous NA reversibly increases PKA activity. These responses were blocked by β-receptors antagonists while a NA transporter inhibitor increased responses. These results demonstrate that endogenous NA released from LC fibers activates the cAMP/PKA pathway in cortical neurons, providing a means to characterize catecholaminergic transmission events." @default.
- W906500352 created "2016-06-24" @default.
- W906500352 creator A5008115457 @default.
- W906500352 date "2014-09-26" @default.
- W906500352 modified "2023-09-25" @default.
- W906500352 title "Imaging of cAMP/PKA dynamics induced by catecholamines in the neocortical network" @default.
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- W906500352 hasPublicationYear "2014" @default.
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