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- W90696492 abstract "Introduction: During August of 2003 an expedition including 19 scientists tested various equipment at a series of hotsprings sites on the island of Svalbard. This island is considered a Mars analogue environment due to the presence of hot springs, carbonate terraces and volcanic activity which have produced carbonate rosettes similar to those found in ALH84001. The goal was to test 4 portable instruments for their robustness as field instruments for life detection (for future human missions to Mars), to assess the Mars analogue environments for signs of life, to refine protocols for contamination reduction and to understand the effects of transport on sample integrity by assessing bioloads immediately in the field and then comparing this with laboratory measurements made after transportation. Materials, Methods and Sites The instruments used in this investigation were; ATP luminometry which assays for ATP as a measure of metabolic activity; Scalar DG2 hand held digital microscope which enables digital microscopy images to be captured at magnifications of up to x200. Charles River Endosafe unit which uses the Limulus Aemebocyte Lysate assay (LAL) and Mobile PCR, which is manufactured by MJ research and is a complete laboratory system containing thermocycler, transilluminator, gel imaging camera system, centrifuge and reagents. Ready mixed reagents (PCR beads) and extraction kits had been previously optimized to ensure contamination free extraction and field PCR. Novel DNA storage and extraction technology (Whatmans FTA paper) was used to supplement traditional techniques (MoBio soil extraction kit). Primer sets concentrated on the use of functional gene detection to characterize the metabolisms present at sites of geochemical interest. This was undertaken so as not to minutely characterize the speciation of the microbial population but to understand what microbial processes were taking place within the environment and to correlate this to" @default.
- W90696492 created "2016-06-24" @default.
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- W90696492 date "2004-03-01" @default.
- W90696492 modified "2023-10-01" @default.
- W90696492 title "In-Field Testing of Life Detection Instruments and Protocols in a Mars Analogue Arctic Environment" @default.
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