Matches in SemOpenAlex for { <https://semopenalex.org/work/W90796720> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W90796720 endingPage "23" @default.
- W90796720 startingPage "18" @default.
- W90796720 abstract "Original modification of the harvest procedure, hypotonic treatment and slide-making techniques were used to obtain prometaphase spreads of good quality fitted to G-banding or to FISH. Human blood cultures were synchronized with a methotrexate block during synthesis, and following this with a thymidine release. Cells were then proceeded into prometaphase and early metaphase and were quickly harvested without exposure to colcemid. Following incubation in a mixture of 0.56% KCl and 1% sodium citrate (1:1) for 20-23 min at room temperature, cells were fixed in 4 changes of fixative made of 3 parts of absolute methanol and 1 part of glacial acetic acid. Three or four 100-105 microliters volume of a suspension of fixed cells were blew with a strength through pasteur pipette tip from a distance 5-6 cm onto chilled superclean slides wetted by water. These slides were then quickly flame dried. Prior to staining, prometaphase bearing spreads were held in thermostat overnight at 37 degrees C. Prometaphases were G-banded with the aid of a modified technique elaborated in the Department of Clinical Genetics, Lund University Hospital (Sweden). The slides were incubated for 1-4 min at room temperature in 0.01 versene solution, containing 0.011% w/v trypsin (Difco), 0.4 mg/ml D-glucose, 0.17 mg/ml KCl, 1.7 mg/ml NaCl. Following a 15 sec incubation in a solution containing 1 mg/ml D-glucose, 0.4 mg/ml KCl, 8 mg/ml NaCl, G-banding was completed by staining the slides with 2% Giemsa (Merck) in pH 6.8 phosphate buffer for 4-5 min.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W90796720 created "2016-06-24" @default.
- W90796720 creator A5004974184 @default.
- W90796720 creator A5013963609 @default.
- W90796720 date "1993-01-01" @default.
- W90796720 modified "2023-09-23" @default.
- W90796720 title "[The variability of the pattern of human G-band chromosomes in the late prophase-early metaphase]." @default.
- W90796720 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8266579" @default.
- W90796720 hasPublicationYear "1993" @default.
- W90796720 type Work @default.
- W90796720 sameAs 90796720 @default.
- W90796720 citedByCount "0" @default.
- W90796720 crossrefType "journal-article" @default.
- W90796720 hasAuthorship W90796720A5004974184 @default.
- W90796720 hasAuthorship W90796720A5013963609 @default.
- W90796720 hasConcept C104317684 @default.
- W90796720 hasConcept C153911025 @default.
- W90796720 hasConcept C154202817 @default.
- W90796720 hasConcept C16685009 @default.
- W90796720 hasConcept C185592680 @default.
- W90796720 hasConcept C25642318 @default.
- W90796720 hasConcept C2780848204 @default.
- W90796720 hasConcept C30481170 @default.
- W90796720 hasConcept C54355233 @default.
- W90796720 hasConcept C55493867 @default.
- W90796720 hasConcept C70278784 @default.
- W90796720 hasConcept C71924100 @default.
- W90796720 hasConcept C74864618 @default.
- W90796720 hasConcept C86803240 @default.
- W90796720 hasConceptScore W90796720C104317684 @default.
- W90796720 hasConceptScore W90796720C153911025 @default.
- W90796720 hasConceptScore W90796720C154202817 @default.
- W90796720 hasConceptScore W90796720C16685009 @default.
- W90796720 hasConceptScore W90796720C185592680 @default.
- W90796720 hasConceptScore W90796720C25642318 @default.
- W90796720 hasConceptScore W90796720C2780848204 @default.
- W90796720 hasConceptScore W90796720C30481170 @default.
- W90796720 hasConceptScore W90796720C54355233 @default.
- W90796720 hasConceptScore W90796720C55493867 @default.
- W90796720 hasConceptScore W90796720C70278784 @default.
- W90796720 hasConceptScore W90796720C71924100 @default.
- W90796720 hasConceptScore W90796720C74864618 @default.
- W90796720 hasConceptScore W90796720C86803240 @default.
- W90796720 hasIssue "9" @default.
- W90796720 hasLocation W907967201 @default.
- W90796720 hasOpenAccess W90796720 @default.
- W90796720 hasPrimaryLocation W907967201 @default.
- W90796720 hasRelatedWork W1974141227 @default.
- W90796720 hasRelatedWork W2018335497 @default.
- W90796720 hasRelatedWork W2037325880 @default.
- W90796720 hasRelatedWork W2038875794 @default.
- W90796720 hasRelatedWork W2043186446 @default.
- W90796720 hasRelatedWork W2047208538 @default.
- W90796720 hasRelatedWork W2338647866 @default.
- W90796720 hasRelatedWork W3181003735 @default.
- W90796720 hasRelatedWork W50207032 @default.
- W90796720 hasRelatedWork W90796720 @default.
- W90796720 hasVolume "35" @default.
- W90796720 isParatext "false" @default.
- W90796720 isRetracted "false" @default.
- W90796720 magId "90796720" @default.
- W90796720 workType "article" @default.