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- W908450917 abstract "The hemagglutinin(HA) gene of Avian Influenza Virus(AIV) subtype H5 and mature chicken interleukin-18(mChIL-18) gene were amplified by designing two pairs of primers according to the sequence published on GenBank.Recombinant baculovirus coexpressing HA1 gene from AIV subtype H5 plus chicken interleukin-18 gene were constructed by inserting cDNA copy of HA gene and cDNA copy of mChIL-18 gene into the baculovirus expression plasmid pFast BacTMDual,furthermore cDNAs respectively inserted into the downstreams of P10 promoter and PH promoter.Finally,the constructing eukaryotic expression plasmid pFastBacTM dual-HA1-mChIL-18 was transposited into Bacterium Coli DH10Bac,which was based on next step insect cell's transfection.The construction of this recombinant plasmid was benefit to investigate the effect of mChIL-18's immunologic enhancement and study a new AIV recombinant vaccine." @default.
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- W908450917 date "2008-01-01" @default.
- W908450917 modified "2023-09-23" @default.
- W908450917 title "Construction of recombinant baculovirus vetor coexpressing HA1 gene from subtype H5 of avian influenza virus and mature chicken interleukin-18 gene" @default.
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