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- W90895039 abstract "The low level of ambient glutamate is extremely important for the brain’s spontaneous activity and proper synaptic transmission. Cholesterol deficiency has been implicated in the pathogenesis of several neurodegenerative disorders. It was examined whether membrane cholesterol modulated the extracellular glutamate level in the nerve terminals and the processes responsible for its maintenance. The ambient l-[14C]glutamate level, being an equilibrium between Na+-dependent uptake and tonic release, was increased from 0.193±0.013nmol/mg protein to 0.282±0.013nmol/mg protein (extracellular endogenous glutamate—from 6.9±2.0nmol/mg protein to 16.6±2.0nmol/mg protein, respectively) in rat brain synaptosomes treated with a cholesterol acceptor MβCD. This alteration was not due to the change in the activity of glutamine synthetase that was shown with the specific blocker l-methionine sulfoximine. Also, cholesterol-deficient synaptosomes showed a lower initial velocity of l-[14C]glutamate uptake." @default.
- W90895039 created "2016-06-24" @default.
- W90895039 creator A5082960233 @default.
- W90895039 date "2013-01-01" @default.
- W90895039 modified "2023-10-16" @default.
- W90895039 title "The Extracellular Level and Uptake of Glutamate in Cholesterol-Deficient Nerve Terminals" @default.
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- W90895039 doi "https://doi.org/10.1007/978-1-4614-7759-4_4" @default.
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