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- W910904734 abstract "将猪流行性腹泻病毒分离株HLJ02,经过胰酶处理后,在Vero细胞上增殖.利用Gen-Bank中的基因序列设计合成了2对M基因引物,应用RT-PCR和RT-nested PCR分别扩增出HLJ02的854 bp的M全基因片段和412 bp的M基因部分片段,而在猪传染性胃肠炎病毒中和Vero培养液中未扩增出上述产物.结果表明,RT-nested PCR可用于检测猪流行性腹泻病毒(PEDV)." @default.
- W910904734 created "2016-06-24" @default.
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- W910904734 date "2003-01-01" @default.
- W910904734 modified "2023-09-23" @default.
- W910904734 title "应用RT—nested PCR检测猪流行性腹泻病毒的研究" @default.
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