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• W91101217 abstract "The use of immature embryo explants of common wheat seems to be the most successful for regeneration and transformation. An alternative explant is the mature embryo, because of the low cost of donor plant production. The main goal of this work was to find less time-consuming transformation protocols, so the whole process of mature embryo transformation in the varieties Bobwhite (GB), Astella (SK) and Mv Csardas (H) was optimised. The best callus induction medium for regeneration was MD 0.5, but the addition of picloram to MS callus induction medium also gave high regeneration and a higher number of shoots in comparison with other auxins. The use of zeatin in combination with 2.4-D auxins led to better regeneration ability and shoot formation. The highest transient and stable gus expression was detected in cv. Mv Csardas on MD 0.5 and 2,4D-CIM callus induction medium" @default.
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• W91101217 date "2011-01-01" @default.
• W91101217 modified "2023-10-18" @default.
• W91101217 title "Agrobacterium-mediated transformation of common wheat (Triticum aestivum L.) using mature embryos." @default.
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