Matches in SemOpenAlex for { <https://semopenalex.org/work/W91342417> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W91342417 endingPage "181.10" @default.
- W91342417 startingPage "181.10" @default.
- W91342417 abstract "Abstract In asthmatic epithelium, the TH2 cytokine milieu results in production of phospholipid-esterified 15-HETE and elevated mucus secretion. A high-throughput 15-HETE binding assay was developed using 15-HETE esterified to biotin and Streptavidin-AlexaFluor488 to form the ligand (15HETE-Bt:SA-AlF). 15HETE-Bt:SA-AlF binding to Beas-2B cells and PBEC grown under air-liquid interface were assessed from 2- 60 min. Specificity of binding was monitored with unlabeled fatty acids [0.05-10 μM], biotin-hydrazide and SA-ALF. CyclicAMP (cAMP) was quantified using a competitive immunoassay kit. Intracellular calcium [Ca2+]i was measured (Fluo-3 / Fura-red) by flow cytometry. Beas-2B (n=8) and PBEC (n=3 donors) demonstrated time-dependent binding of 15HETE-Bt:SA-AlF, reaching equilibrium at 30 min., 4 deg.C. Binding of 15HETE-Bt:SA-AlF was dose-dependently displaced on Beas2B with 15-HETE>12-HETE, 5-HETE > 5,15diHETE,>15-KETE >> LTB4 (p< .05, 15-HETE vs. other fatty acids) whereas biotin-hydrazide:SA-ALF or SA-ALF were ineffective. In Beas2B cells, 15-HETE-Bt [0.1 - 10 μM] induced pM [cAMP] (n =4) whereas cholera toxin pretreatment (1 μg/ml, 5 hr.) inhibited 55-100% of cAMP production (n=2). A transient increase in [Ca2+]i was detected with 3-10 μM 15-HETE-Bt or 15-HETE in Beas2B (n=2). Bronchial epithelial cells exhibit specific, reversible binding of esterified 15-HETE suggesting a unique Gs- GPCR for esterified 15-HETE that may regulate airway inflammation in asthma." @default.
- W91342417 created "2016-06-24" @default.
- W91342417 creator A5003065832 @default.
- W91342417 creator A5006464772 @default.
- W91342417 creator A5036289734 @default.
- W91342417 creator A5045283858 @default.
- W91342417 creator A5060869326 @default.
- W91342417 creator A5067185484 @default.
- W91342417 creator A5090725212 @default.
- W91342417 date "2013-05-01" @default.
- W91342417 modified "2023-09-25" @default.
- W91342417 title "Identification of a G-Protein coupled receptor for esterified 15(S)hydroxyeicosatetraenoic acid on human primary bronchial epithelial cells (P6255)" @default.
- W91342417 doi "https://doi.org/10.4049/jimmunol.190.supp.181.10" @default.
- W91342417 hasPublicationYear "2013" @default.
- W91342417 type Work @default.
- W91342417 sameAs 91342417 @default.
- W91342417 citedByCount "0" @default.
- W91342417 crossrefType "journal-article" @default.
- W91342417 hasAuthorship W91342417A5003065832 @default.
- W91342417 hasAuthorship W91342417A5006464772 @default.
- W91342417 hasAuthorship W91342417A5036289734 @default.
- W91342417 hasAuthorship W91342417A5045283858 @default.
- W91342417 hasAuthorship W91342417A5060869326 @default.
- W91342417 hasAuthorship W91342417A5067185484 @default.
- W91342417 hasAuthorship W91342417A5090725212 @default.
- W91342417 hasConcept C116569031 @default.
- W91342417 hasConcept C153911025 @default.
- W91342417 hasConcept C170493617 @default.
- W91342417 hasConcept C181199279 @default.
- W91342417 hasConcept C185592680 @default.
- W91342417 hasConcept C2778078955 @default.
- W91342417 hasConcept C2779630222 @default.
- W91342417 hasConcept C55493867 @default.
- W91342417 hasConcept C86803240 @default.
- W91342417 hasConceptScore W91342417C116569031 @default.
- W91342417 hasConceptScore W91342417C153911025 @default.
- W91342417 hasConceptScore W91342417C170493617 @default.
- W91342417 hasConceptScore W91342417C181199279 @default.
- W91342417 hasConceptScore W91342417C185592680 @default.
- W91342417 hasConceptScore W91342417C2778078955 @default.
- W91342417 hasConceptScore W91342417C2779630222 @default.
- W91342417 hasConceptScore W91342417C55493867 @default.
- W91342417 hasConceptScore W91342417C86803240 @default.
- W91342417 hasIssue "1_Supplement" @default.
- W91342417 hasLocation W913424171 @default.
- W91342417 hasOpenAccess W91342417 @default.
- W91342417 hasPrimaryLocation W913424171 @default.
- W91342417 hasRelatedWork W1530987995 @default.
- W91342417 hasRelatedWork W2019934305 @default.
- W91342417 hasRelatedWork W2023027368 @default.
- W91342417 hasRelatedWork W2039716262 @default.
- W91342417 hasRelatedWork W2085473671 @default.
- W91342417 hasRelatedWork W2101665346 @default.
- W91342417 hasRelatedWork W2186914726 @default.
- W91342417 hasRelatedWork W2418200004 @default.
- W91342417 hasRelatedWork W3024826994 @default.
- W91342417 hasRelatedWork W89645382 @default.
- W91342417 hasVolume "190" @default.
- W91342417 isParatext "false" @default.
- W91342417 isRetracted "false" @default.
- W91342417 magId "91342417" @default.
- W91342417 workType "article" @default.