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- W91608497 abstract "Photophosphorylation with spinach chloroplast fragments was catalyzed by 3-pyridinealdehyde-DPN and 3-pyridinealdehyde-deamino-DPN. Neither ferredoxin nor ferredoxin-TPN reductase was necessary. During phosphorylation, there was no apparent reduction of the analogs. Furthermore, phosphorylation with the enzy-matically reduced analogs was very low. The oxidized forms also catalyzed an uptake of oxygen with illuminated chloroplast fragments. Hydrolysis of the analogs with phosphodiesterase did not result in a marked decrease in catalysis of phosphorylation. It is concluded that a substituted pyridine ring with a quaternary N-atom is necessary for catalytic activity. The inhibition by CMU and desaspidin was the same as for the photophosphorylation accompanying TPN reduction. A weak EPR signal could be detected with the analogs and illuminated chloroplast fragments. It is suggested that, during photophosphorylation, the analogs are converted only to a radical form and that the electron flow is similar in part to that required for TPN reduction." @default.
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- W91608497 date "1966-07-01" @default.
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- W91608497 title "Photosynthetic reactions with pyridine nucleotide analogs" @default.
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- W91608497 doi "https://doi.org/10.1016/s0003-9861(66)81034-2" @default.
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