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- W91654813 abstract "This chapter focuses on the practical aspects and the uses of adenosine triphosphate (ATP) permeabilization as an investigative tool. Permeabilization of the plasma membrane by extracellular ATP offers a rapid and simple method for introducing small membrane-impermeant molecules into the cytoplasmic matrix of susceptible cells. Mouse macrophages, transformed mouse fibroblasts, rat mast cells, and neuroblastoma cell lines can be permeabilized by ATP, whereas human neutrophils and untransformed fibroblasts cannot. Permeabilization by ATP can be rapidly reversed by removal or chelation of ATP; however, cells may not regain normal morphology and cytosolic ionic composition for 30–60 minutes after reversal of permeabilization. Permeabilization by ATP has already been used successfully to study cell function after introduction of nucleotide analogs, calcium buffers, and fluorescent dyes into the cytoplasmic matrix, and may provide opportunities for similar studies in other cell types as well." @default.
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- W91654813 date "1989-01-01" @default.
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- W91654813 title "Chapter 3 ATP Permeabilization of the Plasma Membrane" @default.
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- W91654813 doi "https://doi.org/10.1016/s0091-679x(08)61601-5" @default.
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