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- W916572576 abstract "In mycobacteria, probing the association of cytoplasmic proteins with the membrane itself, as well as with integral or peripheral membrane proteins, is limited by the difficulty in extracting intact sealed membrane vesicles due to the complex cell wall structure. Here we tested the association of Mycobacterium tuberculosis SecA1 and SecA2 proteins with intact membrane vesicles by a flotation assay using iodixanol density gradients. These protocols have wide applications for studying the association of other mycobacterial cytoplasmic proteins with the membrane and membrane-associated proteins." @default.
- W916572576 created "2016-06-24" @default.
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- W916572576 date "2015-09-01" @default.
- W916572576 modified "2023-09-27" @default.
- W916572576 title "A method to investigate protein association with intact sealed mycobacterial membrane vesicles" @default.
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- W916572576 doi "https://doi.org/10.1016/j.ab.2015.06.023" @default.
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