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- W91665723 abstract "Two lymphoid cell‐specific proteins, called RAG‐1 and RAG‐2, initiate the process of antigen receptor gene rearrangement, termed V(D)J recombination, by assembling a protein–DNA complex with two recombination signal sequences (RSSs), each of which adjoins a different receptor gene segment, and then introducing a DNA double strand break at the end of each RSS. The study of RAG–RSS complex assembly and activity has been facilitated by the development of methods to purify the RAG proteins and members of the HMG‐box family of high mobility group proteins such as HMGB1 that promote RAG binding and cleavage activity in vitro. This chapter describes the purification of recombinant truncated and full‐length RAG‐1 and RAG‐2 expressed transiently in mammalian cells, as well as the purification of bacterially expressed full‐length HMGB1. In addition, it details several experimental procedures used in our laboratory to study RAG–RSS complex formation and function in vitro." @default.
- W91665723 created "2016-06-24" @default.
- W91665723 creator A5018574336 @default.
- W91665723 creator A5021876418 @default.
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- W91665723 date "2006-01-01" @default.
- W91665723 modified "2023-09-26" @default.
- W91665723 title "RAG and HMGB1 Proteins: Purification and Biochemical Analysis of Recombination Signal Complexes" @default.
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- W91665723 doi "https://doi.org/10.1016/s0076-6879(06)08032-3" @default.
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