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- W920441504 abstract "采用RT-PCR技术克隆南极衣藻GR基因ORF全长cDNA,然后经酶切、连接等步骤构建其原核表达载体;并对其表达的诱导时间、IPTG浓度、温度进行了优化,以期获得较大量的酶蛋白。结果表明,将构建的原核表达载体pET-GR导入大肠杆菌BL21,可以高效表达融合蛋白;且表达的蛋白均以包涵体的形式存在;经SDS-PAGE电泳结果显示,获得的目的蛋白分子量为52.2kDa,符合预期分子量;GR蛋白在大肠杆菌中诱导表达优化条件为,1.0mmol/L的IPTG,28℃诱导3h。这些结果为进一步深入研究该基因的特性与功能奠定了基础。" @default.
- W920441504 created "2016-06-24" @default.
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- W920441504 date "2011-01-01" @default.
- W920441504 modified "2023-09-23" @default.
- W920441504 title "南极衣藻(Chlamydomonas sp.ICE-L)谷胱甘肽还原酶基因的原核表达及其条件优化" @default.
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