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- W922903518 abstract "Streptomyces natalensis ATCC27448 produces natamycin, a commercially important macrolide antifungal antibiotic. For molecular genetic study of S. natalensis, we have developed a system for introducing DNA into S. natalensis via conjugal transfer from Escherichia coli. An effective transformation procedure for S. natalensis was established based on transconjugation from E, coli ET12567/pUZ8002 using a -derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 10 mM using of E.coli donor cells without heat treatment of spores. In addition, southern blot analysis of exconjugants and the sequence of plasmids containing DNA flanking the insertion sites from the chromosome revealed that S. natalensis contains a single attB site and at least a secondary or pseudo attB site. Similar to the case of various Streptomyces species, a single attB site of S. natalensis is present within an ORF encoding a pirin-homolog, but a pseudo-attB site is present within a distinct site (GenBank accession no. ) and also its sequence deviates from the consensus sequences of attB sequence." @default.
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- W922903518 date "2005-01-01" @default.
- W922903518 modified "2023-09-25" @default.
- W922903518 title "Transformation using Conjugal Transfer and attB Site Properties of Streptomyces natalensis ATCC27448" @default.
- W922903518 hasPublicationYear "2005" @default.
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