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- W92387145 abstract "Active transport of inorganic phosphate into whole cells of a strain (AB3311) derived from Escherichia coli K12 was found to be partially resistant to 50 μM carbonyl cyanide m-chlorophenyl hydrazone (CCCP), a powerful uncoupler of oxidative phosphorylation. The presence of 10 mM dithiothreitol (DTT) before the addition of CCCP completely prevented the inhibition of phosphate uptake caused by the uncoupler. The addition of DTT to the CCCP-inhibited system restored phosphate uptake to the control rate even when added 5 min after the phosphate transport assay was started. This uncoupler resistant transport is insensitive to anaerobiosis, or the addition of 10 mM KCN which reduces oxygen consumption to less than 1% that of aerobic controls. Additional studies of transport in a mutant (CBT302) deficient in membranebound Ca2+-, Mg2+-ATPase activity also demonstrated the retention of appreciable inorganic phosphate uptake under anaerobic conditions." @default.
- W92387145 created "2016-06-24" @default.
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- W92387145 date "1975-02-01" @default.
- W92387145 modified "2023-09-26" @default.
- W92387145 title "Uncoupler and anaerobic resistant transport of phosphate in Escherichia coli" @default.
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- W92387145 doi "https://doi.org/10.1016/0006-291x(75)90436-2" @default.
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