Matches in SemOpenAlex for { <https://semopenalex.org/work/W92491438> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W92491438 abstract "To deepen our understanding of the regulatory mechanism of smooth muscle contraction, we examined properties of smooth muscle myosin phosphatase (SMMP) which was purified from chicken gizzard according to the method of Alessi et al. with slight modifications. The SMMP was a heterotrimer of 130, 37 and 20 kDa subunits as reported. Because the enzymatic activity was strongly dependent on the ionic strength, all experiments were carried out at a constant ionic strength of 0.15 M. The Vmax and K(m) of the enzyme toward bovine stomach phosphorylated myosin was 25.2 mumol/mg protein/min and 0.45 microM at 25 degrees C, respectively, which implied that the rate of dephosphorylation by SMMP in smooth muscle cells should be comparable to that of phosphorylation by the myosin light chain kinase in the presence of a saturating Ca2+ concentration. ATP dose-dependently decreased the SMMP activity to one-fifth. The IC50 for ATP was 0.6 microM in the presence of 5 mM MgCl2, whereas it increased to 10 microM in the absence of Mg2+ with the addition of 1 mM EDTA. ADP, AMP, GTP and ITP showed no or only weak effect. The finding that ATP gamma S was ineffective indicates that phosphorylation of the 130 kDa subunit cannot be the underlying mechanism. This modulation was observed with myosin from bovine stomach, but not from chicken gizzard. The difference in conformation of myosin molecules in the presence of MgATP may be critical in its modulatory effect." @default.
- W92491438 created "2016-06-24" @default.
- W92491438 creator A5002974385 @default.
- W92491438 creator A5043719742 @default.
- W92491438 date "1998-01-01" @default.
- W92491438 modified "2023-09-27" @default.
- W92491438 title "Modulatory Effect of MgATP on Smooth Muscle Myosin Phosphatase Activity" @default.
- W92491438 cites W1518499796 @default.
- W92491438 cites W1863186034 @default.
- W92491438 cites W1970590142 @default.
- W92491438 cites W1971431976 @default.
- W92491438 cites W1981553500 @default.
- W92491438 cites W1983814120 @default.
- W92491438 cites W2003576865 @default.
- W92491438 cites W2014234136 @default.
- W92491438 cites W2069142867 @default.
- W92491438 cites W2079067261 @default.
- W92491438 cites W2089742414 @default.
- W92491438 cites W2412010214 @default.
- W92491438 cites W2419315415 @default.
- W92491438 cites W2467982037 @default.
- W92491438 cites W2475362550 @default.
- W92491438 cites W2700419483 @default.
- W92491438 cites W2907410636 @default.
- W92491438 cites W2908456606 @default.
- W92491438 cites W3021547911 @default.
- W92491438 doi "https://doi.org/10.1007/978-1-4684-6039-1_28" @default.
- W92491438 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9889835" @default.
- W92491438 hasPublicationYear "1998" @default.
- W92491438 type Work @default.
- W92491438 sameAs 92491438 @default.
- W92491438 citedByCount "1" @default.
- W92491438 crossrefType "book-chapter" @default.
- W92491438 hasAuthorship W92491438A5002974385 @default.
- W92491438 hasAuthorship W92491438A5043719742 @default.
- W92491438 hasConcept C11960822 @default.
- W92491438 hasConcept C12554922 @default.
- W92491438 hasConcept C134018914 @default.
- W92491438 hasConcept C147789679 @default.
- W92491438 hasConcept C178666793 @default.
- W92491438 hasConcept C181199279 @default.
- W92491438 hasConcept C181912034 @default.
- W92491438 hasConcept C184651966 @default.
- W92491438 hasConcept C185592680 @default.
- W92491438 hasConcept C187428577 @default.
- W92491438 hasConcept C2776050358 @default.
- W92491438 hasConcept C2779938346 @default.
- W92491438 hasConcept C55493867 @default.
- W92491438 hasConcept C6997183 @default.
- W92491438 hasConcept C8035138 @default.
- W92491438 hasConcept C86803240 @default.
- W92491438 hasConceptScore W92491438C11960822 @default.
- W92491438 hasConceptScore W92491438C12554922 @default.
- W92491438 hasConceptScore W92491438C134018914 @default.
- W92491438 hasConceptScore W92491438C147789679 @default.
- W92491438 hasConceptScore W92491438C178666793 @default.
- W92491438 hasConceptScore W92491438C181199279 @default.
- W92491438 hasConceptScore W92491438C181912034 @default.
- W92491438 hasConceptScore W92491438C184651966 @default.
- W92491438 hasConceptScore W92491438C185592680 @default.
- W92491438 hasConceptScore W92491438C187428577 @default.
- W92491438 hasConceptScore W92491438C2776050358 @default.
- W92491438 hasConceptScore W92491438C2779938346 @default.
- W92491438 hasConceptScore W92491438C55493867 @default.
- W92491438 hasConceptScore W92491438C6997183 @default.
- W92491438 hasConceptScore W92491438C8035138 @default.
- W92491438 hasConceptScore W92491438C86803240 @default.
- W92491438 hasLocation W924914381 @default.
- W92491438 hasLocation W924914382 @default.
- W92491438 hasOpenAccess W92491438 @default.
- W92491438 hasPrimaryLocation W924914381 @default.
- W92491438 hasRelatedWork W1589149116 @default.
- W92491438 hasRelatedWork W1972331465 @default.
- W92491438 hasRelatedWork W2011472936 @default.
- W92491438 hasRelatedWork W2014956239 @default.
- W92491438 hasRelatedWork W2100619703 @default.
- W92491438 hasRelatedWork W2254386023 @default.
- W92491438 hasRelatedWork W2325931973 @default.
- W92491438 hasRelatedWork W77444976 @default.
- W92491438 hasRelatedWork W802806484 @default.
- W92491438 hasRelatedWork W2013423449 @default.
- W92491438 isParatext "false" @default.
- W92491438 isRetracted "false" @default.
- W92491438 magId "92491438" @default.
- W92491438 workType "book-chapter" @default.