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- W92897770 abstract "Previous studies indicated that prolonged transport stress immediately after mating resulted in a low pregnancy rate (10%). Dietary inclusion of 8% spray-dried plasma (SDP) consistently and markedly increased the pregnancy rate to 49%, and 1% SDP increased it to 35%. We hypothesized that SDP increases pregnancy rate of mated female mice by attenuating inflammation caused by transport stress. Mice (C57BL/6 strain) were shipped from Bar Harbor, ME to Urbana, IL on the day the vaginal plug was found [gestation day (GD) 1], arriving at the laboratory in IL on GD 3. Mice were randomly assigned to diets [0 (CON), 1, or 8% SDP]. Randomly selected mice were euthanized each day from GD 3 to 7 to collect blood and uterus to measure cytokines, Creactive protein (CRP), and cortisol by ELISA and to check pregnancy by inspection. Indicators of inflammation and stress were greatest soon after arrival on GD 3 or 4 (time, P < 0.05). SDP decreased (P < 0.05) uterine TNF-α (45–49%) and IFN-γ (18- 20%), and serum TNF-α (27–41%), CRP (17–19%), and cortisol (8- 12%), compared with CON, but increased (P < 0.05) uterine TGF- β1 (15–16%). SDP markedly increased (P < 0.05) pregnancy rate (176–190%) compared with CON. No difference was found between 1% and 8% SDP. These data support the hypothesis that SDP increases pregnancy rate of mated female mice under these conditions by attenuating inflammation. Grant Funding Source: APC, Inc., Ankeny, IA" @default.
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- W92897770 date "2012-04-01" @default.
- W92897770 modified "2023-09-26" @default.
- W92897770 title "Dietary spray‐dried plasma attenuates inflammation caused by transport stress and increases pregnancy rate of mated female mice" @default.
- W92897770 doi "https://doi.org/10.1096/fasebj.26.1_supplement.1027.3" @default.
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