FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W92985736> ?p ?o ?g. }

• W92985736 abstract "T lymphocytes regulate the initiation, duration, and magnitude of adaptive immune responses and function as effector cells in cell mediated immunity. To become immunologically competent they must generate functional antigen receptors. This process takes place in the thymus and requires somatic recombination of T cell receptor (TCR) genes. It is mediated by the endonucleases recombination activating gene-1 (RAG1) and RAG2. Although the thymus regresses at puberty, T cells are present throughout life implying that other tissues must provide the proper milieu for T cell development. This thesis describes extrathymic T cell maturation in man. RAG1, RAG2, and the preTα-chain (pTα), which is exclusively utilized in developing T cells, were used as markers for TCR gene rearrangement. Two new exons (1A and 1B) encoding sequences in the 5’ untranslated region (5’UTR) of mRNA were discovered in the human RAG1 gene. The previously described 5’UTR exon (renamed 1C) was located between the new exons and exon 2, the latter containing the entire coding sequence. We found that small intestinal lymphocytes of the T cell lineage expressed the new exons in three different splice forms. RAG1 mRNA containing the 1C exon was not expressed in small intestinal lymphocytes. In contrast, splice forms containing the 1A exon were not expressed in thymocytes. RAG1 and pTα mRNA expressing lymphocytes were seen both within the epithelium and in lamina propria. Thymocyte-like CD2+CD7+CD3-, CD4+CD8+, CD1a+, and IL7-R+ lymphocytes were identified in the small intestinal mucosa. CD2+CD7+CD3- cells had the highest expression levels of mRNA for RAG1 and pTα, suggesting that the small intestinal mucosa is indeed a site for T cell maturation. Small intestinal T lymphocytes were also shown to kill via the Fas/FasL pathway in a TCR/CD3 independent manner and via the perforin/granzyme pathway in a TCR/CD3 dependent manner. The Fas/FasL-mediated cytotoxicity may reflect an ongoing selection process of extrathymically maturated T cells.The nasopharyngeal tonsil is the major inductive site for immune reactions against inhaled antigens. Previous demonstration of RAG1 expression in tonsillar B cells was interpreted as antigen driven receptor revision. The present study confirms the expression of RAG1 in B cells. We also found that RAG1, RAG2, and pTa mRNAs were expressed in lymphocytes of the T cell lineage. A small population of cells with the immature phenotype CD2+CD7+CD3- was demonstrated. This population had the highest expression levels of mRNA for RAG1, RAG2, pTα and terminal deoxynucleotidyl transferase. All four splice-forms of RAG1 mRNA were expressed. RAG1 and pTα mRNA expressing cells were mainly located in the proximity of the surface epithelium and in the outer rim of the follicles. These results suggest that the nasopharyngeal tonsil is a site where extrathymic T cell development and antigen driven TCR revision are occurring in parallel.Celiac disease (CD) is a small intestinal enteropathy characterized by permanent intolerance to gluten. Gluten reactive intestinal T cells are central in the pathogenesis and CD can be regarded as a failure to maintain tolerance to this food antigen. Expression of the RAG1 1A/2 splice form was significantly decreased in small intestinal T cell subsets of CD patients suggesting that impaired TCR gene rearrangement could contribute to failure of maintain tolerance in CD.Together, these findings show that both small intestinal and nasopharyngeal tonsillar lymphocytes of T cell lineage have the molecular machinery for antigen receptor rearrangement and that thymocyte-like lymphocytes are present in both tissues. Thus these organs are likely sites of T lymphocyte ontogeny as well as for secondary T cell receptor rearrangement in man." @default.
• W92985736 created "2016-06-24" @default.
• W92985736 creator A5003575996 @default.
• W92985736 creator A5004932750 @default.
• W92985736 creator A5008612449 @default.
• W92985736 creator A5083360665 @default.
• W92985736 date "2003-01-01" @default.
• W92985736 modified "2023-10-18" @default.
• W92985736 title "The nasopharyngeal tonsil - a site for T cell receptor gene rearrangement in man" @default.
• W92985736 hasPublicationYear "2003" @default.
• W92985736 type Work @default.
• W92985736 sameAs 92985736 @default.
• W92985736 citedByCount "0" @default.
• W92985736 crossrefType "journal-article" @default.
• W92985736 hasAuthorship W92985736A5003575996 @default.
• W92985736 hasAuthorship W92985736A5004932750 @default.
• W92985736 hasAuthorship W92985736A5008612449 @default.
• W92985736 hasAuthorship W92985736A5083360665 @default.
• W92985736 hasConcept C104317684 @default.
• W92985736 hasConcept C105580179 @default.
• W92985736 hasConcept C113646796 @default.
• W92985736 hasConcept C114684123 @default.
• W92985736 hasConcept C153911025 @default.
• W92985736 hasConcept C156695909 @default.
• W92985736 hasConcept C157402786 @default.
• W92985736 hasConcept C167672396 @default.
• W92985736 hasConcept C19317047 @default.
• W92985736 hasConcept C2776090121 @default.
• W92985736 hasConcept C2778954852 @default.
• W92985736 hasConcept C2781376893 @default.
• W92985736 hasConcept C2992511266 @default.
• W92985736 hasConcept C36823959 @default.
• W92985736 hasConcept C54355233 @default.
• W92985736 hasConcept C86803240 @default.
• W92985736 hasConcept C8891405 @default.
• W92985736 hasConcept C89604277 @default.
• W92985736 hasConceptScore W92985736C104317684 @default.
• W92985736 hasConceptScore W92985736C105580179 @default.
• W92985736 hasConceptScore W92985736C113646796 @default.
• W92985736 hasConceptScore W92985736C114684123 @default.
• W92985736 hasConceptScore W92985736C153911025 @default.
• W92985736 hasConceptScore W92985736C156695909 @default.
• W92985736 hasConceptScore W92985736C157402786 @default.
• W92985736 hasConceptScore W92985736C167672396 @default.
• W92985736 hasConceptScore W92985736C19317047 @default.
• W92985736 hasConceptScore W92985736C2776090121 @default.
• W92985736 hasConceptScore W92985736C2778954852 @default.
• W92985736 hasConceptScore W92985736C2781376893 @default.
• W92985736 hasConceptScore W92985736C2992511266 @default.
• W92985736 hasConceptScore W92985736C36823959 @default.
• W92985736 hasConceptScore W92985736C54355233 @default.
• W92985736 hasConceptScore W92985736C86803240 @default.
• W92985736 hasConceptScore W92985736C8891405 @default.
• W92985736 hasConceptScore W92985736C89604277 @default.
• W92985736 hasLocation W929857361 @default.
• W92985736 hasOpenAccess W92985736 @default.
• W92985736 hasPrimaryLocation W929857361 @default.
• W92985736 hasRelatedWork W1966070744 @default.
• W92985736 hasRelatedWork W1968926806 @default.
• W92985736 hasRelatedWork W1974306656 @default.
• W92985736 hasRelatedWork W1989875191 @default.
• W92985736 hasRelatedWork W2004037085 @default.
• W92985736 hasRelatedWork W2005150833 @default.
• W92985736 hasRelatedWork W2035122096 @default.
• W92985736 hasRelatedWork W2052115135 @default.
• W92985736 hasRelatedWork W2090210678 @default.
• W92985736 hasRelatedWork W2092680301 @default.
• W92985736 hasRelatedWork W2119237051 @default.
• W92985736 hasRelatedWork W2136314101 @default.
• W92985736 hasRelatedWork W2142463988 @default.
• W92985736 hasRelatedWork W2142778960 @default.
• W92985736 hasRelatedWork W2144422370 @default.
• W92985736 hasRelatedWork W2145568913 @default.
• W92985736 hasRelatedWork W2155090535 @default.
• W92985736 hasRelatedWork W2160789639 @default.
• W92985736 hasRelatedWork W2406631566 @default.
• W92985736 hasRelatedWork W2435859559 @default.
• W92985736 isParatext "false" @default.
• W92985736 isRetracted "false" @default.
• W92985736 magId "92985736" @default.
• W92985736 workType "article" @default.