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- W93157410 abstract "The nucleoside transporter present in chromaffin tissue membranes has been studied by [3H]nitrobenzylthioinosine (NBTI) binding. This ligand presents a high affinity, with a Kd value of 2.1 +/- 0.2 nM and a Bmax of 1.7 +/- 0.2 pmol/mg protein. From the Scatchard and the semilogarithmic graphical representations a positive cooperativity was deduced, with a Hill coefficient of 1.7 +/- 0.4. In displacement studies of NBTI by the non labelled compound, the Hill coefficient was also higher than 1 (1.44 +/- 0.11) in the presence of ATP. This nucleotide seems necessary to maintain the number of high affinity binding sites." @default.
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- W93157410 date "1992-03-01" @default.
- W93157410 modified "2023-10-16" @default.
- W93157410 title "Nitrobenzylthioinosine binding cooperativity in chromaffin tissue membranes." @default.
- W93157410 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1410764" @default.
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