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- W931808125 abstract "AACR Annual Meeting-- Apr 12-16, 2008; San Diego, CA4621 Introduction: HER-2 is an established target for antibody therapy. In vitro priming of T cells with dendritic cells (DCs) pulsed with clinically relevant peptide is an attractive strategy to generate tumor-specific T lymphocyte to activate the host's own immune system to raise an anti-tumor response. We investigated whether tumor specific T cells from umbilical cord blood (UCBs) using autologous DCs pulsed with HER2/neu derived peptide (KIFGSLAFL, E75) could be induced. Methods: UCBs in this experiment were examined with serologic HLA-typing. Only UCBs samples with HLA-A2 and confirmed with HER-2 expression by RT-PCR were used. Immature DCs generated in the presence of interleukin-4 and granulocyte/macrophage colony-stimulating factor from UCBs for 5 days, and then matured with lipopolysaccharide (LPS) and interferon-gamma (IFN-γ). For induction of T cells, DCs were cultured with autologous non-adherent cells and peptide (50μg/ml) was pulsed 3 times, every 2 days. Results: The phenotypes of DCs (CD 80+, CD83, CD 86, and HLA-DR) derived from CD14+ and CD 34+ showed comparable. In vitro primed T cells showed significant peptide-specific IFN-γ cytokine response. Higher expression of CD45RO+ and CD25+ were observed in HER-2/neu specific cytotoxic T lymphocytes when compared with non-activated T cells (20.7%, 36.3% vs. 0.3%, 13.2%). Conclusion: These data suggest that in vitro immunization using DCs could be beneficial in generating tumor specific T cells from Umbilical cord blood, which may be used for adoptive immunotherapy of HER-2 positive breast cancer." @default.
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- W931808125 date "2008-05-01" @default.
- W931808125 modified "2023-10-18" @default.
- W931808125 title "In vitro priming of umbilical cord blood derived T cells using HER-2 peptide pulsed dendritic cells" @default.
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