Matches in SemOpenAlex for { <https://semopenalex.org/work/W932217062> ?p ?o ?g. }
Showing items 1 to 61 of
61
with 100 items per page.
- W932217062 endingPage "379" @default.
- W932217062 startingPage "376" @default.
- W932217062 abstract "目的观察硫酸镍（NiSO4）诱导体外培养大鼠睾丸间质细胞凋亡及其c-kit和caspase-3 mRNA及蛋白表达的变化,探讨镍致睾丸间质细胞损伤的可能机制。方法体外提取和纯化大鼠睾丸间质细胞,用0、250、500和1 000μmol/L NiSO4分别处理睾丸间质细胞,染毒时间分别为6、12和24 h。采用AnnexinV-FITC/PI流式细胞术检测睾丸间质细胞的凋亡发生率,实时荧光定量PCR和Western blotting技术检测c-kit和caspase-3 mRNA及蛋白的表达水平。结果（1）AnnexinV-FITC/PI流式细胞术检测结果显示,间质细胞凋亡主要发生于NiSO41 000μmol/L染毒12 h和NiSO4 250、500、1 000μmol/L染毒24 h组。（2）实时荧光定量PCR结果显示：与对照组比较,染毒6 h NiSO4 250μmol/L、染毒12 hNiSO4 250和500μmol/L c-kit mRNA相对表达量上调（P〈0.05）;染毒24 h NiSO4 500和1 000μmol/L c-kit mRNA相对表达量下降（P〈0.05）。NiSO4各浓度和各时间组caspase-3 mRNA相对表达量均上调（P〈0.05）。（3）Western blotting结果显示：与对照组比较,NiSO4各浓度c-kit蛋白表达逐渐减弱,caspase-3蛋白表达逐渐上调,主要见于NiSO41 000μmol/L染毒6 h以及NiSO4各浓度分别染毒12和24 h。结论 NiSO4可诱导体外大鼠睾丸间质细胞凋亡,且与基因c-kit和caspase-3 mRNA及蛋白异常表达有关。" @default.
- W932217062 created "2016-06-24" @default.
- W932217062 creator A5014861119 @default.
- W932217062 creator A5021011131 @default.
- W932217062 creator A5029919947 @default.
- W932217062 creator A5035933403 @default.
- W932217062 creator A5041284287 @default.
- W932217062 creator A5051761795 @default.
- W932217062 creator A5070750995 @default.
- W932217062 date "2011-10-25" @default.
- W932217062 modified "2023-09-23" @default.
- W932217062 title "硫酸镍诱导体外大鼠睾丸间质细胞凋亡及其对c-kit和caspase-3基因表达的影响" @default.
- W932217062 hasPublicationYear "2011" @default.
- W932217062 type Work @default.
- W932217062 sameAs 932217062 @default.
- W932217062 citedByCount "0" @default.
- W932217062 crossrefType "journal-article" @default.
- W932217062 hasAuthorship W932217062A5014861119 @default.
- W932217062 hasAuthorship W932217062A5021011131 @default.
- W932217062 hasAuthorship W932217062A5029919947 @default.
- W932217062 hasAuthorship W932217062A5035933403 @default.
- W932217062 hasAuthorship W932217062A5041284287 @default.
- W932217062 hasAuthorship W932217062A5051761795 @default.
- W932217062 hasAuthorship W932217062A5070750995 @default.
- W932217062 hasConcept C104317684 @default.
- W932217062 hasConcept C105580179 @default.
- W932217062 hasConcept C12519072 @default.
- W932217062 hasConcept C153911025 @default.
- W932217062 hasConcept C185592680 @default.
- W932217062 hasConcept C190283241 @default.
- W932217062 hasConcept C2775998712 @default.
- W932217062 hasConcept C31573885 @default.
- W932217062 hasConcept C48297814 @default.
- W932217062 hasConcept C55493867 @default.
- W932217062 hasConcept C85265743 @default.
- W932217062 hasConcept C86803240 @default.
- W932217062 hasConcept C98424977 @default.
- W932217062 hasConceptScore W932217062C104317684 @default.
- W932217062 hasConceptScore W932217062C105580179 @default.
- W932217062 hasConceptScore W932217062C12519072 @default.
- W932217062 hasConceptScore W932217062C153911025 @default.
- W932217062 hasConceptScore W932217062C185592680 @default.
- W932217062 hasConceptScore W932217062C190283241 @default.
- W932217062 hasConceptScore W932217062C2775998712 @default.
- W932217062 hasConceptScore W932217062C31573885 @default.
- W932217062 hasConceptScore W932217062C48297814 @default.
- W932217062 hasConceptScore W932217062C55493867 @default.
- W932217062 hasConceptScore W932217062C85265743 @default.
- W932217062 hasConceptScore W932217062C86803240 @default.
- W932217062 hasConceptScore W932217062C98424977 @default.
- W932217062 hasIssue "5" @default.
- W932217062 hasLocation W9322170621 @default.
- W932217062 hasOpenAccess W932217062 @default.
- W932217062 hasPrimaryLocation W9322170621 @default.
- W932217062 hasVolume "25" @default.
- W932217062 isParatext "false" @default.
- W932217062 isRetracted "false" @default.
- W932217062 magId "932217062" @default.
- W932217062 workType "article" @default.