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- W935767428 abstract "Publisher Summary This chapter discusses the assay method and purification of pectinesterases from Phytophthora infestans. The chapter uses simple and less time-consuming method that has been used successfully. Like the titration method it is based on the decrease in pH of the reaction mixture due to increase in free carboxyl groups during enzyme action. The pH optimum can be determined by the titration method. The reaction mixture containing 9 ml of the pectin solution in 0.25 M NaC1 and the enzyme solution is stirred continuously and is kept at the pH to be tested by titration with 0.015 M NaOH. Enzyme activity can be expressed by the amount of NaOH consumed within 30 min at a certain temperature. Pectinesterases of P. infestans are inactivated after l0 min at 60°." @default.
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- W935767428 date "1988-01-01" @default.
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- W935767428 title "Pectinesterases from Phytophthora infestans" @default.
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- W935767428 doi "https://doi.org/10.1016/0076-6879(88)61040-8" @default.
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