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- W935861258 abstract "AIM: To investigate effects of 5-Aza-2'-deoxycitydine (5-Aza-dC) and trichostatin A (TSA) on the expression and methylation of p16 and hMLH-1 gene in gastric carcinoma cells. METHODS: Human gastric cancer cell lines MKN-45 and MGC-803 were cultured in vitro in RPMI 1640 and then treated with different concentrations of 5-Aza-dC and TSA. The methylation of p16 and of hMLH-1 gene in the two kind of cell lines was detected using methylation-specific-polymerase chain reaction (MSP), and the expression levels of p16 and hMLH-1 gene were detected using reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: Reexpression or raised expressions of p16 and hMLH-l mRNA was detected in MGC-803 and MKN-45 gastric cancer cell lines after treatment with 5-Aza-dC alone, or in combination with TSA, or with TSA alone. The two cell lines showed a characteristic DNA methylation status in each promoter region of p16 gene and hMLH-1 gene, p16 gene was hypermethylated in MGC-803 and MKN-45; hMLH-1 gene was hypermethylated in MGC-803, but hemi-methylated in MKN-45. The methylation of p16 and hMLH-1 gene in MKN-45 and MGC-803 cells was reversed after either 5-Aza-dC treatment or TSA treatment. CONCLUSION: Aberrant methylation of p16 and hMLH-1 gene is a common event in the occurrence and progression of gastric cancer. The methylation of promoter region in CPG island is a main cause for p16 and hMLH-1 gene transcriptional inactivation. Siginificantly raised reexpression of methylated tumor suppressor genes was detected after either treatment with 5-Aza-dC alone or in combination with TSA." @default.
- W935861258 created "2016-06-24" @default.
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- W935861258 date "2008-06-18" @default.
- W935861258 modified "2023-09-24" @default.
- W935861258 title "5-Aza-dC和TSA对胃癌细胞系p16和hMLH-1基因甲基化水平及表达的影响" @default.
- W935861258 hasPublicationYear "2008" @default.
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