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- W936008742 abstract "溶葡球菌酶（Lysostaphin）是一种含锌的单链蛋白酶，能有效地杀灭金黄色葡萄球菌。内溶素fEndolysin）是双链DNA噬菌体所特有，是一类胞壁质水解酶，具有广泛的抗菌效果。内溶素与抗生素之间有高效的协同作用。本研究通过BTX电转染和AMAXA核转染的方法将含有溶葡球菌酶（Lysostaphin）和内溶素（Endolysin）两个目的基因（Seq2和Seq3）及绿色荧光蛋白（enhenced green fluorescent protein，EGFP）和新霉素（neomycin，neo）两个标记基因的载体pBCl-seq2＋seq3-EGFP-neo，导入牛BostalxriLs）胎儿成纤维细胞，经荧光观察、G418筛选和PCR鉴定后建立稳定转染的单克隆阳性细胞系。以其作为核供体，体细胞核移植制备转基因胚胎。通过4组不同转染程序（A．023，V-013，V-023和T-016）转染牛胎儿成纤维细胞，结果表明，转染参数T．016最适合本实验，其AMAXA转染效率（20．11％）为BTX电转染转染效率的5倍。转基因囊胚率为（20．08±4．19）％，并发育正常。本研究建立了牛胎儿成纤维细胞系，筛选出高效的转染参数，并获得了含溶葡球菌酶、内溶素的转基因细胞系和转基因囊胚，为制备抗乳房炎转基因牛及寻找防治奶牛乳房炎新途径提供了技术支持。" @default.
- W936008742 created "2016-06-24" @default.
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- W936008742 date "2013-01-01" @default.
- W936008742 modified "2023-09-26" @default.
- W936008742 title "电转染法制备奶牛转溶葡球菌酶（Lysostaphin）和内溶素（Endolysin）基因胚胎" @default.
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