FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W936342002> ?p ?o ?g. }

• W936342002 abstract "Surfactants are compounds that reduce interfacial surface tension, resulting in detergency, emulsifying, foaming and dispersing properties. Surfactants produced via biochemical processes (biosurfactants) form a niche market with their low toxicity, biodegradability and high specificity attributes. Biosurfactants have recently received considerable attention owing to their potential as biomedical molecules. In this study a knowledge base was established for the development of a process which produces biosurfactants for use as antimicrobial agents. Specifically, rhamnolipid biosurfactants were produced from Pseudomonas aeruginosa and tested for antimicrobial activity against target organisms. Accurate and reproducible analyses for the quantification of rhamnolipids and antimicrobial activity were developed. The amount of rhamnolipid was determined indirectly by measuring the rhamnose concentration. A novel HPLC method as well as an orcinol colorimetric method were developed for rhamnose measurement. In order to obtain accuracy with the orcinol method it was found that samples must be extracted at least three times prior to the analysis. An examination of literature on rhamnolipid production showed that many studies used colorimetric methods without extraction. Antibacterial activity was quantified by zone clearing around wells of supernatant in soft agar containing the target organism Mycobacterium aurum. This target organism is especially important in a South African context, since it is used to indicate possible susceptibility of tuberculosis to antibiotics. This method was developed for antibacterial testing, after a standard disk diffusion method proved to be ineffective. Antifungal activity of rhamnolipids was evaluated against the fungus Botrytis cinerea, by growing a lawn of fungus on a plate and adding rhamnolipid. The factors influencing rhamnolipid production were studied by growing different Pseudomonas aeruginosa strains from the ATCC culture collection, namely ATCC 9027 and ATCC 27853 as well as a locally isolated strain under different media conditions. The initial focus was on production of biosurfactants in media containing glucose as substrate. Alkanes were subsequently investigated as an alternative substrate, since they are readily available in South Africa as byproducts from the petrochemical industry. The rhamnolipids produced from the culture collection strains were evaluated for their antibacterial activity against Mycobacterium aurum. A number of key factors were identified which were important for the development of a rhamnolipid production process. Of critical importance were the media conditions. Good production was achieved on glucose media containing a phosphate limitation, pH buffering around neutral pH and a high carbon concentration (2 % carbon). When Pseudomonas aeruginosa ATCC 9027 was cultured on this medium (a minimal salts phosphate limited medium with a Tris buffer), it produced 1.31 g/l rhamnose, equivalent to" @default.
• W936342002 created "2016-06-24" @default.
• W936342002 creator A5058170220 @default.
• W936342002 date "2009-03-01" @default.
• W936342002 modified "2023-09-27" @default.
• W936342002 title "Bacterial production of antimicrobial biosurfactants" @default.
• W936342002 cites W1489730593 @default.
• W936342002 cites W1497309636 @default.
• W936342002 cites W1500059214 @default.
• W936342002 cites W1533818397 @default.
• W936342002 cites W1548069352 @default.
• W936342002 cites W1548740402 @default.
• W936342002 cites W1557995667 @default.
• W936342002 cites W1599937845 @default.
• W936342002 cites W1807136536 @default.
• W936342002 cites W1844469488 @default.
• W936342002 cites W1903990541 @default.
• W936342002 cites W194571274 @default.
• W936342002 cites W1965115595 @default.
• W936342002 cites W1966831828 @default.
• W936342002 cites W1970702862 @default.
• W936342002 cites W1972876611 @default.
• W936342002 cites W1979077916 @default.
• W936342002 cites W1982231336 @default.
• W936342002 cites W1983194020 @default.
• W936342002 cites W1983225740 @default.
• W936342002 cites W1986933355 @default.
• W936342002 cites W1990388805 @default.
• W936342002 cites W1992371971 @default.
• W936342002 cites W1995006590 @default.
• W936342002 cites W2000501692 @default.
• W936342002 cites W2001078382 @default.
• W936342002 cites W2003165281 @default.
• W936342002 cites W2003984067 @default.
• W936342002 cites W2007342922 @default.
• W936342002 cites W2009674410 @default.
• W936342002 cites W2009887687 @default.
• W936342002 cites W2011769869 @default.
• W936342002 cites W2012876904 @default.
• W936342002 cites W2019227237 @default.
• W936342002 cites W2021376949 @default.
• W936342002 cites W2023333050 @default.
• W936342002 cites W2023996371 @default.
• W936342002 cites W2025955985 @default.
• W936342002 cites W2033498639 @default.
• W936342002 cites W2036189834 @default.
• W936342002 cites W2038643168 @default.
• W936342002 cites W2043637175 @default.
• W936342002 cites W2044397973 @default.
• W936342002 cites W2045147306 @default.
• W936342002 cites W2050690244 @default.
• W936342002 cites W2050783180 @default.
• W936342002 cites W2051005210 @default.
• W936342002 cites W2051031814 @default.
• W936342002 cites W2055445906 @default.
• W936342002 cites W2058433465 @default.
• W936342002 cites W2064413035 @default.
• W936342002 cites W2066068744 @default.
• W936342002 cites W2068438477 @default.
• W936342002 cites W2075654276 @default.
• W936342002 cites W2075708702 @default.
• W936342002 cites W2076163247 @default.
• W936342002 cites W2076828703 @default.
• W936342002 cites W2078064123 @default.
• W936342002 cites W2079703899 @default.
• W936342002 cites W2080550202 @default.
• W936342002 cites W2089606209 @default.
• W936342002 cites W2090288316 @default.
• W936342002 cites W2093562729 @default.
• W936342002 cites W2095165256 @default.
• W936342002 cites W2111998755 @default.
• W936342002 cites W2113585081 @default.
• W936342002 cites W2121394375 @default.
• W936342002 cites W2127916045 @default.
• W936342002 cites W2133798991 @default.
• W936342002 cites W2136481800 @default.
• W936342002 cites W2144857074 @default.
• W936342002 cites W2149932166 @default.
• W936342002 cites W2153314075 @default.
• W936342002 cites W2256470077 @default.
• W936342002 cites W2344325115 @default.
• W936342002 cites W2477240654 @default.
• W936342002 cites W626248 @default.
• W936342002 cites W89363244 @default.
• W936342002 hasPublicationYear "2009" @default.
• W936342002 type Work @default.
• W936342002 sameAs 936342002 @default.
• W936342002 citedByCount "4" @default.
• W936342002 countsByYear W9363420022013 @default.
• W936342002 countsByYear W9363420022019 @default.
• W936342002 crossrefType "dissertation" @default.
• W936342002 hasAuthorship W936342002A5058170220 @default.
• W936342002 hasConcept C151730666 @default.
• W936342002 hasConcept C185592680 @default.
• W936342002 hasConcept C2777637488 @default.
• W936342002 hasConcept C2778173381 @default.
• W936342002 hasConcept C2778382314 @default.
• W936342002 hasConcept C2778549369 @default.
• W936342002 hasConcept C2779213145 @default.
• W936342002 hasConcept C2779343474 @default.

• next