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- W936694080 abstract "Publisher Summary This chapter discusses the preparation of labeled pyridine ribonucleotides and ribonucleosides. Nicotinate ribonucleotide (NaMN) and nicotinate adenine dinucleotide (deamido-NAD) is established to be intermediates of NAD biosynthesis both from nicotinate and from tryptophan. Nicotinate-14C ribonucleotide is prepared from commercially available nicotinate-14C and 5-phosphoribosyl-l-pyrophosphate (PRPP) with the use of nicotinate phosphoribosyltransferase. PRPP is prepared from the acetone powder of human erythrocytes or from bakers' yeast. The crude erythrocyte extract also contains deamido-NAD pyrophosphorylase. NaMN labeled with 14C at the ribose portion is prepared from PRPP-14C and nicotinate with yeast nicotinate phosphoribosyltransferase. NaMN-32P is prepared from PRPP-32P and nicotinate. PRPP-32P is prepared from glucose and ATP-γ-32P by the same series of enzymatic reactions as employed in the preparation of NaMN-ribose-14C. NMN-32P is prepared by the use of snake venom phosphodiesterase from NAD-both phosphates-32P isolated from yeast cells grown in a medium containing 32P. Various radioactive nicotinate and nicotinamide ribonucleosides may be prepared from the respective ribonucleotides by removing the phosphate group with either snake venom 5'-nucleotidase, prostatic phosphomonoesterase or Escherichia coli alkaline phosphatase." @default.
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- W936694080 date "1971-01-01" @default.
- W936694080 modified "2023-09-27" @default.
- W936694080 title "[106] Preparation of labeled pyridine ribonucleotides and ribonucleosides" @default.
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- W936694080 doi "https://doi.org/10.1016/s0076-6879(71)18063-9" @default.
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