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- W93711230 abstract "Thesechanges canneither beexplained bythe reduction ofthegeneration time(which isonly10-15%) norfromobserved changes inthereplication timeand inthetimebetween DNA synthesis termination andcell division. Variations were mainly duetotheincrease incell mass perorigin ofreplication, atinitiation, incells grownat39°C. Control ofchromosome replication bytheFplasmid appearstobethereasonfortheincrease intheinitiation mass.Otherpossible causes,such asthemodification ofgrowth temperature, thegeneration time, orboth, were discarded. Theseobservations suggest that atonegrowth rate, theFplasmid replicates ataparticular cell mass toFparticle pumber ratio, andthatthis ratio ishigher thanthecell mass tooriCratio attheinitiation ofchromosome replication. This fact might besignificant tocoordinate thereplication oftwodifferent replicons inthesame cell. Replication ofthechromosome ofEscherichia coliis regulated atthestepofinitiation. Replication isnormally initiated atafixed origin, oriC(2), andproceeds bidirectionally totheterminus region ofthechromosome. Dependence ofinitiation atoriCon dnaAproduct isabsolute (8,22). However, thednaAmutation can besuppressed bythe insertion ofaplasmid into thebacterial chromosome (11, 13, 21). Thereisevidence thatsuchsuppressed strains initiate replication attheplasmid site (3,11,13), whichisinapparent contradiction withthefailure ofinitiation attheplasmid site whenintegrated indnaA+strains. Amodelforthecontrol of replication thatexplains these facts hasbeenproposed by Pritchard etal.(17, 19). Thismodelstates that replicons are controlled bytheir own negatively acting geneproducts. Initiation ofreplication raises theinhibitor concentration, reducing theprobability offurther initiations; growth ofthe hostcell between tworounds ofreplication progressively reduces inhibitor concentration, therefore increasing the probability ofinitiation. A mechanism ofthis typehasbeen demonstrated tocontrol thereplication ofseveral bacterial plasmids, e.g.,ColElandRi(10,12,18). Pritchard's (17) modelalso proposesthatinplasmids abletoproduce integrative suppression, their repressorshould bediluted further thanthechromosomal repressor.Consequently, theseplasmids, whenintegrated indnaA+strains, arepassively replicated as partofthechromosome, withinitiation atthe plasmid site being cancelled. Incontrast, strains lacking the dnaAproduct cancarryoutinitiation attheplasmid site but ata higher cell mass.Totestthishypothesis, we measured thecell mass tochromosome origin ratio atinitiation of replication, theso-called initiation mass (Mi)(7,16)ina dnaAE.coli strain which was suppressed bytheinsertion of theFplasmid into thechromosome. Mivalues arecompared inthisstrain underconditions inwhichreplication iscon" @default.
- W93711230 created "2016-06-24" @default.
- W93711230 creator A5045700150 @default.
- W93711230 date "1986-01-01" @default.
- W93711230 modified "2023-09-27" @default.
- W93711230 title "HostCell Variations Resulting fromFPlasmid-Controlled Replication oftheEscherichia coli Chromosome" @default.
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