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- W937722025 abstract "Publisher Summary The availability of restriction enzymes and cloned nucleotide sequences has made it possible to study plant variation at the level of the genomic DNA. The sensitive tools reveal new types of variability that are not detected by phenotype, karyotype, or enzyme analyses. This chapter discusses the methods for detecting, measuring, and using genetically two kinds of genomic variation that are observed in comparisons of maize inbred lines: quantitative polymorphisms for repeated DNA sequences and sequence variability in the DNA flanking single-copy genes and gene families. To measure quantitative differences in repeated sequences among various DNA samples, it useful to employ a technique of quantitative filter-bound hybridization called “slot blotting.” In constructing genetic linkages, the usefulness of a particular marker depends on the degree of polymorphism at that locus. Restriction fragment length polymorphisms may be frequent within plant genomes and among closely related species so that intraspecific and interspecific crosses will be heterozygous at a large number of loci. As progeny from a single cross can be used to map a large number of gene sequences revealed by hybridization with specific probes, mapped genetic markers can be developed for plant species at a rate that far surpasses that for classical genetic markers." @default.
- W937722025 created "2016-06-24" @default.
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- W937722025 date "1986-01-01" @default.
- W937722025 modified "2023-10-17" @default.
- W937722025 title "Analyzing genome variation in plants" @default.
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- W937722025 doi "https://doi.org/10.1016/0076-6879(86)18065-7" @default.
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