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• W938602276 startingPage "1" @default.
• W938602276 abstract "This chapter discusses tissue culture's role in some selected chemical, pathological, and fundamental aspects of the endocrinology of both steroid and peptide hormone-secreting tissues. Tissue culture has many forms; none is easy and many are both time-consuming and laborious. In some respects, they still represent art forms with a complexity that defies simple analysis. The chapter describes some of the reasons why this has occurred. If, however, tissue culture is to achieve preeminence in experimental studies, its days as an art rather than a science must be numbered. Certain basic problems of methodology, however, still remain to be solved by systematic experimentation, leading ultimately to the preservation of complete structural and functional integrity of purified endocrine cells in culture under completely defined conditions. Further progress in the use of tissue culture to study functional activity and growth may be considerably hampered until these requirements are fulfilled. It would be foolhardy to suppose, however, that there exists one all-encompassing ideal medium or system of culture, and conditions may have to be optimized for each individual cell type or tissue, particularly for human material. Failure to contend with these and similar problems may serve to further perpetuate the myth of culture being inevitably associated with radical changes in the behavior of cells and tissues. A further goal must also be dispensing with the use of serum to sustain cultured cells, as many of the complications that beset endocrine cultures in particular stem from its use. Although efforts in this direction are being made, much remains to be discovered about the precise role played by serum in cultures and the way by which it can be replaced by defined constituents." @default.
• W938602276 created "2016-06-24" @default.
• W938602276 creator A5053332107 @default.
• W938602276 creator A5089169425 @default.
• W938602276 creator A5091856042 @default.
• W938602276 date "1978-01-01" @default.
• W938602276 modified "2023-09-27" @default.
• W938602276 title "Tissue Culture in Endocrine Research: Perspectives, Pitfalls, and Potentials" @default.
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