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- W938611685 abstract "This chapter describes the study demonstrating the purification of commercial NADH. For the purification of 5 g of commercial nicotinamide adenine dinucleotide (NAD)H (Arzneimittelwerk Dresden, G.D.R.) a 5×100 cm column is used. The separation is carried out at 4° in the dark. DEAE-Sephadex A-25, particle size 40-120/μm (Pharmacia, Uppsala, Sweden), is prepared, according to the manufacturer's instructions. It is transformed into the bicarbonate form, by washing, with a 10-fold volume of l M KHCO3, on a sintered-glass funnel layed out with nylon net. The gel is then rinsed, with twice-distilled water, till neutral. The neutral gel is suspended in 50 mM KHCO3 and fine particles are removed by repeated decantations. The prepared gel is poured into the column, with care, taken to avoid turbulences and inclusion of air bubbles. The column outlet is adjusted so that the hydrostatic pressure amounts to 150 cm of water. The gel bed is allowed to stabilize, by running 4 l of 50 mM KHCO3, through the column. The chromatography takes from 5 to 10 days. This chapter discusses the purification of NADH by thin-layer chromatography. Equipment for the removal of the KHCO3 from eluate is described in the chapter. Characterization of purified NADH is also done." @default.
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- W938611685 date "1980-01-01" @default.
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- W938611685 title "[4] Purification of commercial NADH" @default.
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- W938611685 doi "https://doi.org/10.1016/0076-6879(80)66434-9" @default.
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