Matches in SemOpenAlex for { <https://semopenalex.org/work/W938961237> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W938961237 endingPage "1864" @default.
- W938961237 startingPage "1859" @default.
- W938961237 abstract "利用含羰基化合物与2,4-二硝基苯肼在酸性条件下反应得到的产物腙对紫外-可见光有吸收的特性,采用柱前衍生高效液相色谱法测定了1-脱氧-D-木酮糖-5-磷酸合酶稳态动力学参数。酶反应产物1-脱氧-D-木酮糖-5-磷酸在碱性磷酸酶的作用下生成去磷酸化产物1-脱氧-D-木酮糖,然后与2,4-二硝基苯肼衍生成腙。衍生化反应的适宜条件为：HClO4浓度为1.5%,反应温度37℃,反应时间60 min,2,4-二硝基苯肼与1-脱氧-D-木酮糖-5-磷酸的摩尔比为6∶1。色谱条件：0～17 min,40%～80%甲醇;18～20 min,40%甲醇。结果表明,本方法具有较高的灵敏度和良好的线性关系,1-脱氧-D-木酮糖-5-磷酸的检出限为1.0 mg/L,在0.005～1.0 g/L范围内线性相关系数为0.999,相对标准偏差小于5.0%（n=3）,标准回收率为102.22%。用本方法测得的1-脱氧-D-木酮糖-5-磷酸合酶稳态动力学参数Km、Vmax和Kcat与文献报道一致。" @default.
- W938961237 created "2016-06-24" @default.
- W938961237 creator A5022912656 @default.
- W938961237 creator A5026125339 @default.
- W938961237 creator A5030353781 @default.
- W938961237 creator A5056744279 @default.
- W938961237 date "2013-07-30" @default.
- W938961237 modified "2023-09-25" @default.
- W938961237 title "Determination of Steady-State Kinetic Parameters of 1-Deoxy-<I>D</I>-xylulose-5-phosphate Synthase by Pre-column Derivatization High Performance Liquid Chromatography Using 2,4 Dinitrophenylhydrazine as Derivative Reagent" @default.
- W938961237 doi "https://doi.org/10.3724/sp.j.1096.2012.20447" @default.
- W938961237 hasPublicationYear "2013" @default.
- W938961237 type Work @default.
- W938961237 sameAs 938961237 @default.
- W938961237 citedByCount "0" @default.
- W938961237 crossrefType "journal-article" @default.
- W938961237 hasAuthorship W938961237A5022912656 @default.
- W938961237 hasAuthorship W938961237A5026125339 @default.
- W938961237 hasAuthorship W938961237A5030353781 @default.
- W938961237 hasAuthorship W938961237A5056744279 @default.
- W938961237 hasConcept C106159729 @default.
- W938961237 hasConcept C111771559 @default.
- W938961237 hasConcept C162324750 @default.
- W938961237 hasConcept C178790620 @default.
- W938961237 hasConcept C179998833 @default.
- W938961237 hasConcept C185592680 @default.
- W938961237 hasConcept C2776923573 @default.
- W938961237 hasConcept C2777132085 @default.
- W938961237 hasConcept C40875361 @default.
- W938961237 hasConcept C43617362 @default.
- W938961237 hasConcept C8171440 @default.
- W938961237 hasConceptScore W938961237C106159729 @default.
- W938961237 hasConceptScore W938961237C111771559 @default.
- W938961237 hasConceptScore W938961237C162324750 @default.
- W938961237 hasConceptScore W938961237C178790620 @default.
- W938961237 hasConceptScore W938961237C179998833 @default.
- W938961237 hasConceptScore W938961237C185592680 @default.
- W938961237 hasConceptScore W938961237C2776923573 @default.
- W938961237 hasConceptScore W938961237C2777132085 @default.
- W938961237 hasConceptScore W938961237C40875361 @default.
- W938961237 hasConceptScore W938961237C43617362 @default.
- W938961237 hasConceptScore W938961237C8171440 @default.
- W938961237 hasIssue "12" @default.
- W938961237 hasLocation W9389612371 @default.
- W938961237 hasOpenAccess W938961237 @default.
- W938961237 hasPrimaryLocation W9389612371 @default.
- W938961237 hasRelatedWork W1968781128 @default.
- W938961237 hasRelatedWork W2008937680 @default.
- W938961237 hasRelatedWork W2020460345 @default.
- W938961237 hasRelatedWork W2026578023 @default.
- W938961237 hasRelatedWork W2027971524 @default.
- W938961237 hasRelatedWork W2040655948 @default.
- W938961237 hasRelatedWork W2043579647 @default.
- W938961237 hasRelatedWork W2058020531 @default.
- W938961237 hasRelatedWork W2089074688 @default.
- W938961237 hasRelatedWork W2379473750 @default.
- W938961237 hasVolume "40" @default.
- W938961237 isParatext "false" @default.
- W938961237 isRetracted "false" @default.
- W938961237 magId "938961237" @default.
- W938961237 workType "article" @default.