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- W939172625 abstract "Publisher Summary Chloroplasts have been isolated (see Gorham 1) for three principal purposes: (1) studies on electron transport, photophosphorylation, and oxygen evolution in the presence of oxidants other than CO 2 ; (2) carbon assimilation; and (3) examination of enzymic and structural components. The purpose for which the chloroplasts are to be used governs the material, the extraction, and the assay. In the course of preparation, spinach ( Spinacea oleracea ) is favored because in many latitudes, it is freely available for most of the year, it is readily macerated, its vacuoles are largely free of endogenous inhibitors, and it often contains only small quantities of starch and calcium oxalate. For these reasons, it normally yields chloroplasts of exceptionally high activity, which behave in a predictable and relatively reproducible fashion. Higher yields of intact chloroplasts may be obtained by using less conventional blendors, such as the Polytron. Only intact chloroplasts support carbon assimilation and the reduction of 3-phosphoglycerate at appreciable rates. For maximal rates of electron transport, cyclic, and noncyclic photophosphorylation and for obtaining the largest light-reduced pH shifts and some other phenomena, it is necessary to work with envelope-free chloroplasts or lamellar bodies." @default.
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- W939172625 date "1971-01-01" @default.
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- W939172625 title "[18] Chloroplasts (and Grana): Aqueous (including high carbon fixation ability)" @default.
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- W939172625 doi "https://doi.org/10.1016/s0076-6879(71)23094-9" @default.
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