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- W941833150 abstract "根据禽网状内皮组织增生症病毒（REV）长末端重复序列LTR基因保守区域设计并合成一对引物，建立基于SYBRGreenI模式的实时荧光PCR方法（Real—time PCR）。以常规PcR产物为标准品建立标准曲线，并对方法的特异性、敏感性、可重复性以及再现性、检出率进行评价。结果表明。该方法只从REV阳性样本检出扩增信号．不与其它病原出现交叉反应；熔解温度为86．8＋0、5℃。无引物二聚体；扩增产物片段大小为270bp，与预期大小相符。测序结果证实为REV靶序列；在1．01×10^2-1．01×10^9拷贝之间呈现良好的线性关系，相关系数为0．999，扩增效率为99．7％。最低可检测101拷贝／反应阳性标准品。比常规PCR敏感103倍；组内变异系数、组间变异系数分别为4．80％-5．72％、0．75％-3．87％；对16例临床疑似病例进行检测。阳性率为81．25％（13／16），随机抽取7份阳性扩增产物进行测序．证实为REV序列。本研究建立的SYBRGreenI实时荧光PCR特异性强、通量高、灵敏度高、检测速度快．为REV的快速检测、分子流行病学调查以及监测REV污染疫苗情况提供新方法。" @default.
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- W941833150 date "2012-05-02" @default.
- W941833150 modified "2023-09-24" @default.
- W941833150 title "SYBR Green I实时荧光PCR检测网状内皮增生症病毒方法的建立" @default.
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