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- W942354527 abstract "[Objective] Based on optimizing a variety of experimental conditions,a method for the determination of resveratrol in RHIZOMA ET RADIX POLYGONI CUSPIDATI by HPLC was established by using ethanol as extractant.[Method] Phenomenex C18(250 mm×4.6 mm,5 μm) was selected as column,with methanol-0.25% acetic acid(65∶ 35,V/V) as mobile phase,0.8 ml/min mobile speed and 306 nm detection wavelength.Taking the extraction rate of resveratrol as the evaluation index,single factor test and L25(54) orthogonal test were used to optimize the experimental conditions.[Result] The extraction conditions for resveratrol were 60% ethanol,1∶ 30(g/ml) solid-liquid ratio,55 ℃ extraction temperature and 15 min extraction time.Under this condictions,the extraction rate of resveratrol reached 0.826 μg/g.[Conclusion] This method was proved to be simple,rapid,accurate and could be used for the determination of resveratrol content in RHIZOMA ET RADIX POLYGONI CUSPIDATI." @default.
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- W942354527 date "2012-01-01" @default.
- W942354527 modified "2023-09-28" @default.
- W942354527 title "Determination of resveratrol content in RHIZOMA ET RADIX POLYGONI CUSPIDATI by HPLC." @default.
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