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- W94272365 abstract "This chapter discusses the quantification of RNA targets using the polymerase chain reaction (PCR). PCR is the method of choice for the detection of viral nucleic acids present in small amounts in biological samples. In recent years, major improvements in the sensitivity and specificity of PCR methodology have permitted routine diagnosis of viral infections with a high degree of accuracy in clinical laboratories. In virology, quantitative evaluation of viral target sequences is necessary in two major applications, including complex pathogenesis problems and the efficacy of antiviral therapies. In pathogenesis, the goal is to obtain an estimate of viral burden that is as close as possible to the real value (also referred to as absolute quantification). An accurate estimation of the amount of human immunodeficiency virus type 1 (HIV-1) that is present in an infected individual sheds light on the disease process. The chapter discusses the ability of the technologies to provide absolute quantification for pathogenicity studies and precise quantification for therapeutic monitoring. The development and validation of a quantitative HIV-1 RNA PCR assay for the monitoring of anti-HIV-1 immunotherapy is presented." @default.
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- W94272365 date "1995-01-01" @default.
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- W94272365 title "Quantification of RNA Targets Using the Polymerase Chain Reaction" @default.
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- W94272365 doi "https://doi.org/10.1016/b978-012748920-9/50010-8" @default.
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