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- W944704162 abstract "This chapter focuses on three methods for studying neutral connections in mammals. The first and the oldest method involves injection of a radioactive amino acid, [3H]proline, which is incorporated into protein and carried anterogradely to label axons, terminals, and in specific cases, second-order neurons. The second method is labeling with the lectin wheat germ agglutinin conjugated to the marker enzyme horseradish peroxidase (WGA-HRP); while this lectin conjugate is primarily carried anterogradely, there is also the potential for transcellular and retrograde filling of cell bodies. The third method involves fluorescent carbocyanine dyes as both retrograde and anterograde tracers. All these methods have been used in a number of laboratories in the past and the chapter also provides details regarding these methods. The autoradiographic method using 3H-labeled amino acids provides a stable, relatively permanent “photographic” record of the pathway. However, it also has some limitations. WGA-HRP is now commonly used and has proved successful in tracing pathways in a variety of species, in a variety of systems, and in animals ranging in age from fetal to adult. It can be successfully combined with several other techniques, including other tracers, autoradiography, and immunocytochemisty. The chapter further highlights the advantages and limitations of pathway tracing with carbocyanine dyes." @default.
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- W944704162 date "1990-01-01" @default.
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- W944704162 title "Methods for Analyzing Neuronal Connections in Mammals" @default.
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- W944704162 doi "https://doi.org/10.1016/b978-0-12-185255-9.50015-8" @default.
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