FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W94482989> ?p ?o ?g. }

• W94482989 endingPage "236" @default.
• W94482989 startingPage "219" @default.
• W94482989 abstract "In microbial ecology, high-resolution molecular approaches are essential for characterizing the vast organismal and functional diversity and understanding the interaction of microbial communities with biotic and abiotic environmental factors. Integrated omics, comprising genomics, transcriptomics, proteomics, and metabolomics allows conclusive links to be drawn between genetic potential and function. However, this requires truly systematic measurements. In this chapter, we first assess the levels of heterogeneity within mixed microbial communities, thereby demonstrating the need for analyzing biomolecular fractions obtained from a single and undivided sample to facilitate multi-omic analysis and meaningful data integration. Further, we describe a methodological workflow for the reproducible isolation of concomitant metabolites, RNA (optionally split into large and small RNA fractions), DNA, and proteins. Depending on the nature of the sample, the methodology comprises different (pre)processing and preservation steps. If possible, extracellular polar and nonpolar metabolites may first be extracted from cell supernatants using organic solvents. Cells are homogenized by cryomilling before small molecules are extracted with organic solvents. After cell lysis, nucleic acids and protein fractions are sequentially isolated using chromatographic spin columns. To prove the broad applicability of the methodology, we applied it to microbial consortia of biotechnological (biological wastewater treatment biomass), environmental (freshwater planktonic communities), and biomedical (human fecal sample) research interest. The methodological framework should be applicable to other microbial communities as well as other biological samples with a minimum of tailoring and represents an important first step in standardization for the emerging field of Molecular Eco-Systems Biology." @default.
• W94482989 created "2016-06-24" @default.
• W94482989 creator A5042972806 @default.
• W94482989 creator A5065171942 @default.
• W94482989 creator A5075385734 @default.
• W94482989 creator A5076380000 @default.
• W94482989 date "2013-01-01" @default.
• W94482989 modified "2023-10-18" @default.
• W94482989 title "Sequential Isolation of Metabolites, RNA, DNA, and Proteins from the Same Unique Sample" @default.
• W94482989 cites W1967551069 @default.
• W94482989 cites W1978460879 @default.
• W94482989 cites W1981385227 @default.
• W94482989 cites W1990126728 @default.
• W94482989 cites W1993026417 @default.
• W94482989 cites W2053929618 @default.
• W94482989 cites W2060811342 @default.
• W94482989 cites W2080283023 @default.
• W94482989 cites W2091523617 @default.
• W94482989 cites W2101962198 @default.
• W94482989 cites W2113813261 @default.
• W94482989 cites W2137548031 @default.
• W94482989 cites W2154273881 @default.
• W94482989 cites W4294216491 @default.
• W94482989 doi "https://doi.org/10.1016/b978-0-12-407863-5.00011-3" @default.
• W94482989 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24060123" @default.
• W94482989 hasPublicationYear "2013" @default.
• W94482989 type Work @default.
• W94482989 sameAs 94482989 @default.
• W94482989 citedByCount "51" @default.
• W94482989 countsByYear W944829892014 @default.
• W94482989 countsByYear W944829892015 @default.
• W94482989 countsByYear W944829892016 @default.
• W94482989 countsByYear W944829892017 @default.
• W94482989 countsByYear W944829892018 @default.
• W94482989 countsByYear W944829892019 @default.
• W94482989 countsByYear W944829892020 @default.
• W94482989 countsByYear W944829892021 @default.
• W94482989 countsByYear W944829892022 @default.
• W94482989 countsByYear W944829892023 @default.
• W94482989 crossrefType "book-chapter" @default.
• W94482989 hasAuthorship W94482989A5042972806 @default.
• W94482989 hasAuthorship W94482989A5065171942 @default.
• W94482989 hasAuthorship W94482989A5075385734 @default.
• W94482989 hasAuthorship W94482989A5076380000 @default.
• W94482989 hasConcept C104317684 @default.
• W94482989 hasConcept C127413603 @default.
• W94482989 hasConcept C132215390 @default.
• W94482989 hasConcept C141231307 @default.
• W94482989 hasConcept C183696295 @default.
• W94482989 hasConcept C18903297 @default.
• W94482989 hasConcept C189206191 @default.
• W94482989 hasConcept C21565614 @default.
• W94482989 hasConcept C2775941552 @default.
• W94482989 hasConcept C46111723 @default.
• W94482989 hasConcept C523546767 @default.
• W94482989 hasConcept C54355233 @default.
• W94482989 hasConcept C55493867 @default.
• W94482989 hasConcept C57409179 @default.
• W94482989 hasConcept C60644358 @default.
• W94482989 hasConcept C67705224 @default.
• W94482989 hasConcept C69562835 @default.
• W94482989 hasConcept C70721500 @default.
• W94482989 hasConcept C86803240 @default.
• W94482989 hasConceptScore W94482989C104317684 @default.
• W94482989 hasConceptScore W94482989C127413603 @default.
• W94482989 hasConceptScore W94482989C132215390 @default.
• W94482989 hasConceptScore W94482989C141231307 @default.
• W94482989 hasConceptScore W94482989C183696295 @default.
• W94482989 hasConceptScore W94482989C18903297 @default.
• W94482989 hasConceptScore W94482989C189206191 @default.
• W94482989 hasConceptScore W94482989C21565614 @default.
• W94482989 hasConceptScore W94482989C2775941552 @default.
• W94482989 hasConceptScore W94482989C46111723 @default.
• W94482989 hasConceptScore W94482989C523546767 @default.
• W94482989 hasConceptScore W94482989C54355233 @default.
• W94482989 hasConceptScore W94482989C55493867 @default.
• W94482989 hasConceptScore W94482989C57409179 @default.
• W94482989 hasConceptScore W94482989C60644358 @default.
• W94482989 hasConceptScore W94482989C67705224 @default.
• W94482989 hasConceptScore W94482989C69562835 @default.
• W94482989 hasConceptScore W94482989C70721500 @default.
• W94482989 hasConceptScore W94482989C86803240 @default.
• W94482989 hasLocation W944829891 @default.
• W94482989 hasLocation W944829892 @default.
• W94482989 hasOpenAccess W94482989 @default.
• W94482989 hasPrimaryLocation W944829891 @default.
• W94482989 hasRelatedWork W2007091097 @default.
• W94482989 hasRelatedWork W2017773668 @default.
• W94482989 hasRelatedWork W2039940120 @default.
• W94482989 hasRelatedWork W2108939148 @default.
• W94482989 hasRelatedWork W2133826923 @default.
• W94482989 hasRelatedWork W2134884997 @default.
• W94482989 hasRelatedWork W2261819947 @default.
• W94482989 hasRelatedWork W2391375401 @default.
• W94482989 hasRelatedWork W2909698402 @default.
• W94482989 hasRelatedWork W2189119546 @default.
• W94482989 isParatext "false" @default.
• W94482989 isRetracted "false" @default.

• next